Title |
Engineering of a high lipid producing Yarrowia lipolytica strain
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Published in |
Biotechnology for Biofuels and Bioproducts, March 2016
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DOI | 10.1186/s13068-016-0492-3 |
Pubmed ID | |
Authors |
Jonathan Friedlander, Vasiliki Tsakraklides, Annapurna Kamineni, Emily H. Greenhagen, Andrew L. Consiglio, Kyle MacEwen, Donald V. Crabtree, Jonathan Afshar, Rebecca L. Nugent, Maureen A. Hamilton, A. Joe Shaw, Colin R. South, Gregory Stephanopoulos, Elena E. Brevnova |
Abstract |
Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. The yields, productivities, and titers reported in this study may further support the applied goal of cost-effective, large -scale microbial lipid production for use as biofuels and biochemicals. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Sweden | 2 | <1% |
United States | 1 | <1% |
Unknown | 248 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 47 | 19% |
Researcher | 40 | 16% |
Student > Master | 32 | 13% |
Student > Bachelor | 23 | 9% |
Student > Doctoral Student | 15 | 6% |
Other | 30 | 12% |
Unknown | 64 | 25% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 60 | 24% |
Biochemistry, Genetics and Molecular Biology | 59 | 24% |
Engineering | 18 | 7% |
Chemical Engineering | 13 | 5% |
Environmental Science | 8 | 3% |
Other | 18 | 7% |
Unknown | 75 | 30% |