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Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis

Overview of attention for article published in Gut Pathogens, April 2016
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Title
Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis
Published in
Gut Pathogens, April 2016
DOI 10.1186/s13099-016-0094-4
Pubmed ID
Authors

Hongxia Wang, Baowei Diao, Zhigang Cui, Meiying Yan, Biao Kan

Abstract

Typhoid fever has caused severe epidemics in many Asian and African countries. The early detection of outbreaks and their sources may promote the prevention and control of typhoid fever, for which effective and timely molecular subtyping techniques are required. Pulsed field gel electrophoresis (PFGE) is routinely used as the molecular typing technique for foodborne and waterborne pathogens. However, maneuverable techniques remain necessary to expedite the experimental procedure and obtain more effective subtyping. The multilocus loci of a variable number of tandem repeats (VNTR) analysis (MLVA) is a polymerase chain reaction (PCR)-based subtyping method. MLVA method and PFGE based on Xba I enzyme were applied to the 103 Salmonella Typhi (S. Typhi) isolated from different years and regions. Dendrograms were constructed and analyzed to help understand the data. The Simpson's index of diversity (D value) was calculated to estimate the discriminatory power of MLVA and PFGE. In addition, a set of endogenous 3 bp DNA ladder markers were established to accurately determine the repeat copy number of the VNTR with only a 3 bp repetitive unit, using microfluidics chip-based electrophoresis to generate comparable VNTR data in the public health laboratory network. The established 8-loci MLVA for S. Typhi subtyping had higher discriminatory power than PFGE. In some cases, PFGE could not distinguish the strains isolated over long intervals and with different epidemic provinces. By contrast, 8-loci MLVA distinctly distinguished these strains, and the strains with the same MLVA patterns were from the same or contiguous years and the same province, showing its significance in epidemiological discrimination. The established set of endogenous 3 bp DNA ladder markers improved the accuracy and reproducibility of VNTR analysis using microfluidics chip-based electrophoresis to 100 %. Eight VNTRs can be used for the MLVA analysis of the 103 S. Typhi isolates. MLVA based on the 8-loci had higher discriminatory power than PFGE for S. Typhi subtyping. The 8-loci MLVA is easier for the analysis and interpretation of relationships between strains compared to PFGE.

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 31%
Student > Postgraduate 2 13%
Researcher 2 13%
Other 1 6%
Professor 1 6%
Other 3 19%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 38%
Immunology and Microbiology 3 19%
Medicine and Dentistry 2 13%
Linguistics 1 6%
Biochemistry, Genetics and Molecular Biology 1 6%
Other 1 6%
Unknown 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 April 2016.
All research outputs
#5,670,496
of 7,492,829 outputs
Outputs from Gut Pathogens
#143
of 196 outputs
Outputs of similar age
#191,315
of 272,154 outputs
Outputs of similar age from Gut Pathogens
#7
of 13 outputs
Altmetric has tracked 7,492,829 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 196 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.