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Filming a live cell by scanning electrochemical microscopy: label-free imaging of the dynamic morphology in real time

Overview of attention for article published in BMC Chemistry, March 2012
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Title
Filming a live cell by scanning electrochemical microscopy: label-free imaging of the dynamic morphology in real time
Published in
BMC Chemistry, March 2012
DOI 10.1186/1752-153x-6-20
Pubmed ID
Authors

Michelle Meng-Ni Zhang, Yi-Tao Long, Zhifeng Ding

Abstract

The morphology of a live cell reflects the organization of the cytoskeleton and the healthy status of the cell. We established a label-free platform for monitoring the changing morphology of live cells in real time based on scanning electrochemical microscopy (SECM). The dynamic morphology of a live human bladder cancer cell (T24) was revealed by time-lapse SECM with dissolved oxygen in the medium solution as the redox mediator. Detailed local movements of cell membrane were presented by time-lapse cross section lines extracted from time-lapse SECM. Vivid dynamic morphology is presented by a movie made of time-lapse SECM images. The morphological change of the T24 cell by non-physiological temperature is in consistence with the morphological feature of early apoptosis. To obtain dynamic cellular morphology with other methods is difficult. The non-invasive nature of SECM combined with high resolution realized filming the movements of live cells.

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Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
United States 1 3%
Unknown 34 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 44%
Student > Master 5 14%
Student > Bachelor 3 8%
Student > Doctoral Student 2 6%
Professor 2 6%
Other 4 11%
Unknown 4 11%
Readers by discipline Count As %
Chemistry 17 47%
Agricultural and Biological Sciences 6 17%
Biochemistry, Genetics and Molecular Biology 2 6%
Physics and Astronomy 2 6%
Chemical Engineering 1 3%
Other 5 14%
Unknown 3 8%