Title |
Filming a live cell by scanning electrochemical microscopy: label-free imaging of the dynamic morphology in real time
|
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Published in |
BMC Chemistry, March 2012
|
DOI | 10.1186/1752-153x-6-20 |
Pubmed ID | |
Authors |
Michelle Meng-Ni Zhang, Yi-Tao Long, Zhifeng Ding |
Abstract |
The morphology of a live cell reflects the organization of the cytoskeleton and the healthy status of the cell. We established a label-free platform for monitoring the changing morphology of live cells in real time based on scanning electrochemical microscopy (SECM). The dynamic morphology of a live human bladder cancer cell (T24) was revealed by time-lapse SECM with dissolved oxygen in the medium solution as the redox mediator. Detailed local movements of cell membrane were presented by time-lapse cross section lines extracted from time-lapse SECM. Vivid dynamic morphology is presented by a movie made of time-lapse SECM images. The morphological change of the T24 cell by non-physiological temperature is in consistence with the morphological feature of early apoptosis. To obtain dynamic cellular morphology with other methods is difficult. The non-invasive nature of SECM combined with high resolution realized filming the movements of live cells. |
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Germany | 1 | 50% |
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Scientists | 1 | 50% |
Mendeley readers
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Unknown | 34 | 94% |
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Student > Ph. D. Student | 16 | 44% |
Student > Master | 5 | 14% |
Student > Bachelor | 3 | 8% |
Student > Doctoral Student | 2 | 6% |
Professor | 2 | 6% |
Other | 4 | 11% |
Unknown | 4 | 11% |
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Chemical Engineering | 1 | 3% |
Other | 5 | 14% |
Unknown | 3 | 8% |