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Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, April 2016
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Title
Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance
Published in
Biotechnology for Biofuels and Bioproducts, April 2016
DOI 10.1186/s13068-016-0497-y
Pubmed ID
Authors

Bo Hu, Yi-Ming Yang, David A. C. Beck, Qian-Wen Wang, Wen-Jing Chen, Jing Yang, Mary E. Lidstrom, Song Yang

Abstract

The toxicity of alcohols is one of the major roadblocks of biological fermentation for biofuels production. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from cheap carbon feedstocks through a synthetic metabolic pathway. However, M. extorquens AM1 is vulnerable to solvent stress, which impedes further development for 1-butanol production. Only a few studies have reported the general stress response of M. extorquens AM1 to solvent stress. Therefore, it is highly desirable to obtain a strain with ameliorated 1-butanol tolerance and elucidate the molecular mechanism of 1-butnaol tolerance in M. extorquens AM1 for future strain improvement. In this work, adaptive laboratory evolution was used as a tool to isolate mutants with 1-butanol tolerance up to 0.5 %. The evolved strains, BHBT3 and BHBT5, demonstrated increased growth rates and higher survival rates with the existence of 1-butanol. Whole genome sequencing revealed a SNP mutation at kefB in BHBT5, which was confirmed to be responsible for increasing 1-butanol tolerance through an allelic exchange experiment. Global metabolomic analysis further discovered that the pools of multiple key metabolites, including fatty acids, amino acids, and disaccharides, were increased in BHBT5 in response to 1-butanol stress. Additionally, the carotenoid synthesis pathway was significantly down-regulated in BHBT5. We successfully screened mutants resistant to 1-butanol and provided insights into the molecular mechanism of 1-butanol tolerance in M. extorquens AM1. This research will be useful for uncovering the mechanism of cellular response of M. extorquens AM1 to solvent stress, and will provide the genetic blueprint for the rational design of a strain of M. extorquens AM1 with increased 1-butanol tolerance in the future.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Belgium 1 2%
Unknown 64 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 20%
Researcher 11 17%
Student > Master 8 12%
Professor > Associate Professor 4 6%
Student > Bachelor 4 6%
Other 11 17%
Unknown 15 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 38%
Biochemistry, Genetics and Molecular Biology 14 21%
Engineering 6 9%
Chemical Engineering 4 6%
Business, Management and Accounting 1 2%
Other 2 3%
Unknown 14 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2016.
All research outputs
#17,285,036
of 25,371,288 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#997
of 1,578 outputs
Outputs of similar age
#194,168
of 316,329 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#31
of 42 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,329 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.