Title |
One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3
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Published in |
BMC Veterinary Research, March 2012
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DOI | 10.1186/1746-6148-8-37 |
Pubmed ID | |
Authors |
Leenadevi Thonur, Madeleine Maley, Janice Gilray, Tara Crook, Ellie Laming, Dylan Turnbull, Mintu Nath, Kim Willoughby |
Abstract |
Detection of respiratory viruses in veterinary species has traditionally relied on virus detection by isolation or immunofluorescence and/or detection of circulating antibody using ELISA or serum neutralising antibody tests. Multiplex real time PCR is increasingly used to diagnose respiratory viruses in humans and has proved to be superior to traditional methods. Bovine respiratory disease (BRD) is one of the most common causes of morbidity and mortality in housed cattle and virus infections can play a major role. We describe here a one step multiplex reverse transcriptase quantitative polymerase chain reaction (mRT-qPCR) to detect the viruses commonly implicated in BRD. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Indonesia | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 90 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 23% |
Researcher | 12 | 13% |
Student > Master | 7 | 8% |
Student > Doctoral Student | 6 | 7% |
Professor | 5 | 5% |
Other | 17 | 18% |
Unknown | 24 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 28% |
Veterinary Science and Veterinary Medicine | 14 | 15% |
Biochemistry, Genetics and Molecular Biology | 8 | 9% |
Medicine and Dentistry | 6 | 7% |
Unspecified | 3 | 3% |
Other | 7 | 8% |
Unknown | 28 | 30% |