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Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, April 2016
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Title
Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
Published in
Biotechnology for Biofuels and Bioproducts, April 2016
DOI 10.1186/s13068-016-0503-4
Pubmed ID
Authors

Nadin Pade, Sabrina Erdmann, Heike Enke, Frederik Dethloff, Ulf Dühring, Jens Georg, Juliane Wambutt, Joachim Kopka, Wolfgang R. Hess, Ralf Zimmermann, Dan Kramer, Martin Hagemann

Abstract

Cyanobacteria are phototrophic prokaryotes that convert inorganic carbon as CO2 into organic compounds at the expense of light energy. They need only inorganic nutrients and can be cultivated to high densities using non-arable land and seawater. This has made cyanobacteria attractive organisms for the production of biofuels and chemical feedstock. Synechocystis sp. PCC 6803 is one of the most widely used cyanobacterial model strains. Based on its available genome sequence and genetic tools, Synechocystis has been genetically modified to produce different biotechnological products. Efficient isoprene production is an attractive goal because this compound is widely used as chemical feedstock. Here, we report on our attempts to generate isoprene-producing strains of Synechocystis using a plasmid-based strategy. As previously reported, a codon-optimized plant isoprene synthase (IspS) was expressed under the control of different Synechocystis promoters that ensure strong constitutive or light-regulated ispS expression. The expression of the ispS gene was quantified by qPCR and Western blotting, while the amount of isoprene was quantified using GC-MS. In addition to isoprene measurements in the headspace of closed culture vessels, single photon ionization time-of-flight mass spectrometry (SPI-MS) was applied, which allowed online measurements of isoprene production in open-cultivation systems under various conditions. Under standard conditions, a good correlation existed between ispS expression and isoprene production rate. The cultivation of isoprene production strains under NaCl-supplemented conditions decreased isoprene production despite enhanced ispS mRNA levels. The characterization of the metabolome of isoprene-producing strains indicated that isoprene production might be limited by insufficient precursor levels. Transcriptomic analysis revealed the upregulation of mRNA and regulatory RNAs characteristic of acclimation to metabolic stress. Our best production strains produced twofold higher isoprene amounts in the presence of low NaCl concentrations than previously reported strains. These results will guide future attempts to establish isoprene production in cyanobacterial hosts.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 <1%
Brazil 1 <1%
Unknown 110 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 20%
Researcher 19 17%
Student > Bachelor 16 14%
Student > Master 12 11%
Student > Doctoral Student 7 6%
Other 14 13%
Unknown 22 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 37 33%
Agricultural and Biological Sciences 33 29%
Engineering 6 5%
Environmental Science 3 3%
Chemistry 3 3%
Other 7 6%
Unknown 23 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2016.
All research outputs
#16,047,334
of 25,373,627 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#881
of 1,578 outputs
Outputs of similar age
#171,986
of 313,519 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#25
of 39 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,519 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.