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Molecular characterization of Hepatitis C virus 3a in Peshawar

Overview of attention for article published in BMC Infectious Diseases, April 2016
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Title
Molecular characterization of Hepatitis C virus 3a in Peshawar
Published in
BMC Infectious Diseases, April 2016
DOI 10.1186/s12879-016-1488-0
Pubmed ID
Authors

Amina Gul, Nabeela Zahid, Jawad Ahmed, Fazli Zahir, Imtiaz Ali Khan, Ijaz Ali

Abstract

The purpose of this study was to explore molecular epidemiology of HCV genotype 3a in Peshawar based on sequencing and phylogenetic analysis of Core region of HCV genome. Chronically infected Hepatitis C virus infected patients enrolled under the Prime Minister Hepatitis C control program at three Tertiary care units of Peshawar [Khyber Teaching Hospital Peshawar, Lady Reading Hospital Peshawar, Hayat Abad Medical Complex Peshawar] were included in this cross sectional observational study. Qualitative detection of HCV and HCV genotyping was carried out by a modified reverse transcription-polymerase chain reaction (RT-PCR) and type specific genotyping assay. The Core gene of HCV genotype 3a was amplified, cloned and sequenced. The sequences obtained were used for phylogenetic analysis using MEGA 6 software. Among the 422 (82.75 %) PCR positive samples, 192 (45.5 %) were identified as having HCV genotype 3a infection. HCV Core gene sequencing was carried out randomly for the characterization of HCV 3a. Nucleotide sequence analysis of the obtained viral genomic sequences based on partial HCV 3a Core gene sequences with reference sequences from different countries showed that our sequences clustered with some local and regional sequences with high bootstrap values. HCV 3a is highly prevalent in Peshawar, Pakistan and its phylogenetics based on Core gene sequences indicate the prevalence of different lineages of HCV 3a in Peshawar which may have consequences for disease management strategies causing more economic pressure on the impoverished population due to possible antiviral resistance.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 22%
Professor > Associate Professor 5 19%
Researcher 3 11%
Student > Bachelor 3 11%
Other 1 4%
Other 2 7%
Unknown 7 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 30%
Biochemistry, Genetics and Molecular Biology 7 26%
Medicine and Dentistry 2 7%
Social Sciences 1 4%
Nursing and Health Professions 1 4%
Other 0 0%
Unknown 8 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2016.
All research outputs
#20,322,106
of 22,865,319 outputs
Outputs from BMC Infectious Diseases
#6,477
of 7,687 outputs
Outputs of similar age
#253,403
of 299,111 outputs
Outputs of similar age from BMC Infectious Diseases
#100
of 115 outputs
Altmetric has tracked 22,865,319 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,687 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,111 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 115 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.