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Evaluation of the in vitro expression of ATP binding-cassette (ABC) proteins in an Ixodes ricinus cell line exposed to ivermectin

Overview of attention for article published in Parasites & Vectors, April 2016
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Mentioned by

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2 tweeters

Citations

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16 Dimensions

Readers on

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30 Mendeley
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2 CiteULike
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Title
Evaluation of the in vitro expression of ATP binding-cassette (ABC) proteins in an Ixodes ricinus cell line exposed to ivermectin
Published in
Parasites & Vectors, April 2016
DOI 10.1186/s13071-016-1497-2
Pubmed ID
Authors

Carlo Mangia, Alice Vismarra, Laura Kramer, Lesley Bell-Sakyi, Daniele Porretta, Domenico Otranto, Sara Epis, Giulio Grandi

Abstract

Ticks are among the most important vectors of pathogens causing human and animal disease. Acaricides are used to control tick infestation, although there are increasing reports of resistance. Recently, over-expression of ATP-binding cassette (ABC) transporter proteins (P-glycoproteins, PgP) has been implicated in resistance to the acaricide ivermectin in the ticks Rhipicephalus (Boophilus) microplus and Rhipicephalus sanguineus sensu lato. Ixodid tick cell lines have been used to investigate drug resistance mechanisms. The aim of the present study was to evaluate expression of several PgPs in the Ixodes ricinus-derived cell line IRE/CTVM19 and to determine modulation of expression following treatment with ivermectin. IRE/CTVM19 cells were treated with different concentrations of ivermectin (0, 11, 22 or 33 μM) and incubated for 10 days. Evaluation of viability and relative expression of ABCB1, ABCB6, ABCB8 and ABCB10 genes were carried out at day 10 post treatment. Cell viability ranged between 84 % and 92 % with no significant differences between untreated and treated cells. qRT-PCR showed that ABC pump expression was not significantly modulated by ivermectin treatment. Expression of the ABCB8 PgP subfamily revealed a biphasic trend, based on the ivermectin concentration. ABCB6 and ABCB10 gene expression was not modulated by ivermectin treatment and ABCB1 expression was not detected. This is the first report of PgP expression in an I. ricinus-derived tick cell line. Development of an in vitro model for the study of acaricide resistance mechanisms would greatly facilitate screening for drug resistance in ticks.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 27%
Student > Doctoral Student 4 13%
Student > Master 4 13%
Student > Ph. D. Student 3 10%
Student > Bachelor 2 7%
Other 4 13%
Unknown 5 17%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 5 17%
Biochemistry, Genetics and Molecular Biology 5 17%
Agricultural and Biological Sciences 4 13%
Medicine and Dentistry 2 7%
Engineering 2 7%
Other 6 20%
Unknown 6 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2016.
All research outputs
#3,893,186
of 7,584,550 outputs
Outputs from Parasites & Vectors
#1,144
of 2,081 outputs
Outputs of similar age
#143,518
of 267,005 outputs
Outputs of similar age from Parasites & Vectors
#102
of 186 outputs
Altmetric has tracked 7,584,550 research outputs across all sources so far. This one is in the 48th percentile – i.e., 48% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,081 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,005 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 186 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.