@Lovin_Chromatin @KurianLab @selifeski I get it :) my guess would be that if the entire protocol is done at cold temperature, the impact would be minimal. Lower temp also helps with reducing the impact of tissue digestion. Hence, cold digestive enzymes are
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we found this ref that uses a cold-active protease called subtilisin A, has anyone tried it? https://t.co/zgOCfzOCGG
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@MadS100tist https://t.co/bQz89Vc8Rm This helped a lot. There are new class of cold enzymes that would be great to try, but haven't got a chance: https://t.co/h0o50o0DYt
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