Report for: Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

Title	Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism
Published in	BMC Chemistry, April 2012
DOI	10.1186/1752-153x-6-29
Pubmed ID	22490336
Authors	Chin-Chuan Wei, Nicole Reynolds, Christina Palka, Kristen Wetherell, Tiffany Boyle, Ya-Ping Yang, Zhi-Qiang Wang, Dennis J Stuehr
Abstract	Superoxide generated by non-phagocytic NADPH oxidases (NOXs) is of growing importance for physiology and pathobiology. The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. To better understand the metal binding properties of the 1st and 2nd EF-hands, we characterized the N-terminal half of CaBD (NCaBD) and its calcium-binding knockout mutants.

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Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism