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Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates from a tertiary hospital in the Philippines

Overview of attention for article published in Tropical Medicine and Health, March 2016
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Title
Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates from a tertiary hospital in the Philippines
Published in
Tropical Medicine and Health, March 2016
DOI 10.1186/s41182-016-0003-z
Pubmed ID
Authors

Demetrio L. Valle, Phyllis Anne P. Paclibare, Esperanza C. Cabrera, Windell L. Rivera

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to public health worldwide. There are relatively few studies addressing the molecular epidemiology of MRSA in the Philippines. This study characterized MRSA isolates in terms of their antimicrobial susceptibility profile, the SCCmec type, and the presence of lukF-lukS genes for Panton-Valentine leukocidin (PVL) and determined the relatedness of the isolates by random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR). A total of 236 S. aureus were isolated from clinical specimens of the Makati Medical Center in Makati City, Philippines, between January 2013 and June 2013, and 108 or 45.76 % were found to be MRSA. Results showed that the MRSA strains were resistant to trimethoprim-sulfamethoxazole (20.37 %), azithromycin (10.19 %), gentamicin (5.56 %), and linezolid (4.63 %), while all were susceptible to vancomycin, nitrofurantoin, levofloxacin, minocycline, rifampin, and tetracycline. One isolate was found positive for inducible clindamycin resistance. All of the 108 MRSA strains were confirmed to carry the mecA and SCCmec genes, while the PVL genes were detected in 41 (38 %) of the isolates. Ninety-six isolates (89 %) carried SCCmec type IV, while the remaining isolates carried SCCmec type I (11 isolates) or type III (one isolate). This study is the first to present a comprehensive MRSA surveillance data with molecular characterization in a tertiary hospital in the Philippines.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Philippines 1 1%
India 1 1%
Unknown 84 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 14%
Student > Bachelor 12 14%
Student > Postgraduate 8 9%
Researcher 6 7%
Librarian 4 5%
Other 13 15%
Unknown 31 36%
Readers by discipline Count As %
Immunology and Microbiology 14 16%
Agricultural and Biological Sciences 10 12%
Medicine and Dentistry 10 12%
Biochemistry, Genetics and Molecular Biology 9 10%
Nursing and Health Professions 3 3%
Other 7 8%
Unknown 33 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2016.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from Tropical Medicine and Health
#222
of 441 outputs
Outputs of similar age
#181,882
of 314,537 outputs
Outputs of similar age from Tropical Medicine and Health
#7
of 9 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 441 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,537 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.