@AwfulDodger @lpachter @NAR_Open @badlytrainedtec It's very challenging to do compositional normalization at single-cell (not pseudobulk) level. TMM and median-of-ratios can't be applied when almost everything you have are zeroes or ones. Good discussion h
247 followers
5 followers
@lpachter @sinabooeshaghi @IngileifBryndis @agalvezmerchan And what about the scran normalization? https://t.co/RscDyEBhMG
1,098 followers
@hummuscience @satijalab @wolfgangkhuber I like this paper (https://t.co/cYvWXg3BCJ) by Aaron Lun et al. for calculating the size factors of single cell datasets.
2,989 followers
Next up Catalina Vallejos @CataVallejosM Showed how to normalize scRNAseq data using RPM, DESeq, TMM. Cited Aaron Lun (@bioconductor developer) https://t.co/IRPKpeskQl https://t.co/xdchevdB2l and her work with @drisso1893 Introducing BASiCS #LatinInSTEM