↓ Skip to main content

The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Overview of attention for article published in Plant Methods, April 2016
Altmetric Badge

Mentioned by

twitter
2 X users

Citations

dimensions_citation
13 Dimensions

Readers on

mendeley
23 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
Published in
Plant Methods, April 2016
DOI 10.1186/s13007-016-0124-8
Pubmed ID
Authors

Petr Cápal, Takashi R. Endo, Jan Vrána, Marie Kubaláková, Miroslava Karafiátová, Eva Komínková, Isabel Mora-Ramírez, Winfriede Weschke, Jaroslav Doležel

Abstract

Identification of transgene insertion sites in plant genomes has practical implications for crop breeding and is a stepping stone to analyze transgene function. However, single copy sequences are not always easy to localize in large plant genomes by standard approaches. We employed flow cytometric chromosome sorting to determine chromosomal location of barley sucrose transporter construct in three transgenic lines of common wheat. Flow-sorted chromosomes were used as template for PCR and fluorescence in situ hybridization to identify chromosomes with transgenes. The chromosomes carrying the transgenes were then confirmed by PCR using DNA amplified from single flow-sorted chromosomes as template. Insertion sites of the transgene were unambiguously localized to chromosomes 4A, 7A and 5D in three wheat transgenic lines. The procedure presented in this study is applicable for localization of any single-copy sequence not only in wheat, but in any plant species where suspension of intact mitotic chromosomes suitable for flow cytometric sorting can be prepared.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 35%
Student > Ph. D. Student 5 22%
Student > Doctoral Student 3 13%
Student > Master 2 9%
Student > Bachelor 1 4%
Other 1 4%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 65%
Biochemistry, Genetics and Molecular Biology 4 17%
Unknown 4 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2016.
All research outputs
#18,453,763
of 22,865,319 outputs
Outputs from Plant Methods
#959
of 1,083 outputs
Outputs of similar age
#218,571
of 298,657 outputs
Outputs of similar age from Plant Methods
#8
of 9 outputs
Altmetric has tracked 22,865,319 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,083 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 3rd percentile – i.e., 3% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,657 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one.