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Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9

Overview of attention for article published in Journal of Biological Engineering, April 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#14 of 283)
  • High Attention Score compared to outputs of the same age (93rd percentile)

Mentioned by

news
2 news outlets
blogs
1 blog
twitter
2 X users
patent
2 patents
wikipedia
5 Wikipedia pages

Citations

dimensions_citation
36 Dimensions

Readers on

mendeley
142 Mendeley
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Title
Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9
Published in
Journal of Biological Engineering, April 2016
DOI 10.1186/s13036-016-0028-1
Pubmed ID
Authors

Damien Biot-Pelletier, Vincent J. J. Martin

Abstract

CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mutations. To fulfill this promise, a strategy that effects single nucleotide changes while preventing repeated recognition and cutting by the gRNA/Cas9 complex is needed. We demonstrate a two-step method to introduce point mutations at 17 positions in the S. cerevisiae genome. We show the general applicability of the method, enabling the seamless introduction of single nucleotide changes at any location, including essential genes and non-coding regions. We also show a quantifiable phenotype for a point mutation introduced in gene GSH1. The ease and wide applicability of this general method, combined with the demonstration of its feasibility will enable genome editing at an unprecedented level of detail in yeast and other organisms.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 142 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
India 1 <1%
China 1 <1%
Unknown 138 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 29 20%
Student > Ph. D. Student 24 17%
Student > Bachelor 22 15%
Student > Master 12 8%
Other 9 6%
Other 23 16%
Unknown 23 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 55 39%
Agricultural and Biological Sciences 43 30%
Engineering 7 5%
Chemical Engineering 4 3%
Medicine and Dentistry 3 2%
Other 4 3%
Unknown 26 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 32. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2023.
All research outputs
#1,106,309
of 23,806,312 outputs
Outputs from Journal of Biological Engineering
#14
of 283 outputs
Outputs of similar age
#19,932
of 300,833 outputs
Outputs of similar age from Journal of Biological Engineering
#1
of 3 outputs
Altmetric has tracked 23,806,312 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 283 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.5. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,833 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them