Title |
Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system
|
---|---|
Published in |
BMC Biotechnology, May 2012
|
DOI | 10.1186/1472-6750-12-18 |
Pubmed ID | |
Authors |
Tereza C Cardoso, Heitor F Ferrari, Andrea F Garcia, Juliana B Novais, Camila Silva-Frade, Marina C Ferrarezi, Alexandre L Andrade, Roberto Gameiro |
Abstract |
The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D) environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). The three-dimensional (3D) cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Wharton's jelly (UC-WJ) cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | <1% |
France | 1 | <1% |
Brazil | 1 | <1% |
Iran, Islamic Republic of | 1 | <1% |
Sri Lanka | 1 | <1% |
United States | 1 | <1% |
Jordan | 1 | <1% |
Unknown | 113 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 21 | 18% |
Student > Master | 19 | 16% |
Student > Ph. D. Student | 15 | 13% |
Student > Bachelor | 11 | 9% |
Student > Doctoral Student | 9 | 8% |
Other | 22 | 18% |
Unknown | 23 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 30 | 25% |
Biochemistry, Genetics and Molecular Biology | 21 | 18% |
Medicine and Dentistry | 21 | 18% |
Veterinary Science and Veterinary Medicine | 8 | 7% |
Immunology and Microbiology | 3 | 3% |
Other | 10 | 8% |
Unknown | 27 | 23% |