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The screening and functional study of proteins binding with the BmNPV polyhedrin promoter

Overview of attention for article published in Virology Journal, May 2012
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Title
The screening and functional study of proteins binding with the BmNPV polyhedrin promoter
Published in
Virology Journal, May 2012
DOI 10.1186/1743-422x-9-90
Pubmed ID
Authors

Wei Yu, Jia Li, Meihui Wang, Yanping Quan, Jian Chen, Zuoming Nie, Zhengbing Lv, Yaozhou Zhang

Abstract

The polyhedrin gene promoter has an essential role in regulating foreign gene expression in baculovirus expression vector systems (BEVS); however, the high-level transcription mechanism is still unknown. One-hybrid screening in yeast is a powerful way of identifying rapidly heterologous transcription factors that can interact with the polyhedrin promoter DNA sequence. In the current study, total RNA was extracted from the fat bodies of fifth-instar silkworm larvae that had been infected with Bombyx mori nuclear polyhedrosis virus (BmNPV) for 5 days; complementary DNA (cDNA) was then generated using reverse-transcription (RT)-PCR to construct a silkworm gene expression library. Key polyhedrin promoter bait sequences were synthesized to generate a bait yeast strain, which was used to screen the one-hybrid cDNA library.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 31%
Student > Ph. D. Student 3 19%
Student > Master 3 19%
Student > Bachelor 1 6%
Student > Postgraduate 1 6%
Other 0 0%
Unknown 3 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 63%
Biochemistry, Genetics and Molecular Biology 3 19%
Unknown 3 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 May 2012.
All research outputs
#18,305,773
of 22,664,644 outputs
Outputs from Virology Journal
#2,420
of 3,029 outputs
Outputs of similar age
#126,291
of 163,548 outputs
Outputs of similar age from Virology Journal
#26
of 47 outputs
Altmetric has tracked 22,664,644 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,029 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.5. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
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We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.