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The Tetrahymena metallothionein gene family: twenty-one new cDNAs, molecular characterization, phylogenetic study and comparative analysis of the gene expression under different abiotic stressors

Overview of attention for article published in BMC Genomics, May 2016
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Title
The Tetrahymena metallothionein gene family: twenty-one new cDNAs, molecular characterization, phylogenetic study and comparative analysis of the gene expression under different abiotic stressors
Published in
BMC Genomics, May 2016
DOI 10.1186/s12864-016-2658-6
Pubmed ID
Authors

Patricia de Francisco, Laura María Melgar, Silvia Díaz, Ana Martín-González, Juan Carlos Gutiérrez

Abstract

Ciliate metallothioneins (MTs) are included in family 7 of the MT superfamily. This family has been divided into two main subfamilies: 7a or CdMTs and 7b or CuMTs. All ciliate MTs reported have been isolated from different Tetrahymena species and present unique features with regard to standard MTs. Likewise, an expression analysis has been carried out on some of MT genes under metal stress, corroborating their classification into two subfamilies. We isolated 21 new cDNAs from different Tetrahymena species to obtain a wider view of the biodiversity of these conserved genes. Structural analysis (cysteine patterns) and an updated phylogenetic study both corroborated the previous classification into two subfamilies. A new CuMT from a Tetrahymena-related species Ichthyophthirius multifiliis was also included in this general analysis. We detected a certain tendency towards the presentation of a CdMT tri-modular structure in Borealis group species with respect to Australis group. We report for the first time a semi-complete paralog duplication of a CdMT gene originating a new CdMT gene isoform in T. malaccensis. An asymmetry of the codon usage for glutamine residues was detected between Cd- and CuMTs, and the phylogenetic implications are discussed. A comparative gene expression analysis of several MT genes by qRT-PCR revealed differential behavior among them under different abiotic stressors in the same Tetrahymena species. The Tetrahymena metallothionein family represents a quite conserved protein structure group with unique features with respect to standard MTs. Both Cd- and CuMT subfamilies present very defined and differentiated characteristics at several levels: cysteine patterns, modular structure, glutamine codon usage and gene expression under metal stress, among others. Gene duplication through evolution seems to be the major genetic mechanism for creating new MT gene isoforms and increasing their functional diversity.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 15%
Student > Ph. D. Student 5 15%
Student > Bachelor 4 12%
Researcher 3 9%
Lecturer 1 3%
Other 4 12%
Unknown 11 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 24%
Biochemistry, Genetics and Molecular Biology 5 15%
Environmental Science 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Business, Management and Accounting 1 3%
Other 3 9%
Unknown 13 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2021.
All research outputs
#18,456,836
of 22,869,263 outputs
Outputs from BMC Genomics
#8,191
of 10,664 outputs
Outputs of similar age
#224,358
of 304,990 outputs
Outputs of similar age from BMC Genomics
#168
of 198 outputs
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