Title |
Exploring molecular evolution of Rubisco in C3 and CAM Orchidaceae and Bromeliaceae
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Published in |
BMC Ecology and Evolution, January 2020
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DOI | 10.1186/s12862-019-1551-8 |
Pubmed ID | |
Authors |
Carmen Hermida-Carrera, Mario A. Fares, Marcel Font-Carrascosa, Maxim V. Kapralov, Marcus A. Koch, Arnau Mir, Arántzazu Molins, Miquel Ribas-Carbó, Jairo Rocha, Jeroni Galmés |
Abstract |
The CO2-concentrating mechanism associated to Crassulacean acid metabolism (CAM) alters the catalytic context for Rubisco by increasing CO2 availability and provides an advantage in particular ecological conditions. We hypothesized about the existence of molecular changes linked to these particular adaptations in CAM Rubisco. We investigated molecular evolution of the Rubisco large (L-) subunit in 78 orchids and 144 bromeliads with C3 and CAM photosynthetic pathways. The sequence analyses were complemented with measurements of Rubisco kinetics in some species with contrasting photosynthetic mechanism and differing in the L-subunit sequence. We identified potential positively selected sites and residues with signatures of co-adaptation. The implementation of a decision tree model related Rubisco specific variable sites to the leaf carbon isotopic composition of the species. Differences in the Rubisco catalytic traits found among C3 orchids and between strong CAM and C3 bromeliads suggested Rubisco had evolved in response to differing CO2 concentration. The results revealed that the variability in the Rubisco L-subunit sequence in orchids and bromeliads is composed of coevolving sites under potential positive adaptive signal. The sequence variability was related to δ13C in orchids and bromeliads, however it could not be linked to the variability found in the kinetic properties of the studied species. |
X Demographics
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Spain | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 6 | 17% |
Student > Master | 6 | 17% |
Student > Ph. D. Student | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Researcher | 3 | 8% |
Other | 4 | 11% |
Unknown | 10 | 28% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 17% |
Environmental Science | 5 | 14% |
Social Sciences | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 0 | 0% |
Unknown | 10 | 28% |