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Virulence and resistance features of Pseudomonas aeruginosa strains isolated from chronic leg ulcers

Overview of attention for article published in BMC Infectious Diseases, March 2016
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Title
Virulence and resistance features of Pseudomonas aeruginosa strains isolated from chronic leg ulcers
Published in
BMC Infectious Diseases, March 2016
DOI 10.1186/s12879-016-1396-3
Pubmed ID
Authors

Mihaela Georgescu, Irina Gheorghe, Carmen Curutiu, Veronica Lazar, Coralia Bleotu, Mariana-Carmen Chifiriuc

Abstract

The purpose of this study was to evaluate the virulence profiles of Pseudomonas aeruginosa clinical strains recently isolated from patients hospitalized for chronic leg ulcers in the Dermatology Department of Central Military Emergency University Hospital "Carol Davila", Bucharest, Romania. The phenotypic screening evaluated eight soluble virulence factors (haemolysins, lecithinase, lipase, caseinase, gelatinase, amylase, DNase, aesculin hydrolysis), as well as adherence ability (Cravioto adapted method) and invasion capacity on HeLa cells (gentamicin protection assay). Seven virulence genes encoding for protease IV, 3 exoenzymes (exoS, exoT, exoU), two phospholipases plcH- haemolytic phospholipase C and plcN- non-haemolytic phospholipase C) and alginate were investigated by PCR. The pore forming toxins and enzymes were expressed in variable proportions, the majority of the tested strains producing beta haemolysin (92.3 %), lipase (76.9 %) and lecithinase (61.5 %). The most frequent virulence genes detected in the analyzed strains were the ExoT (100 %) and AlgD (92.3 %) genes, genes codifying for phospholipases (84.6 % each of them) and for protease IV (61.5 %). This study reveals that correlating virulence profiles and infection clinical outcome is very useful for setting up efficient preventive and therapeutic procedures for hospitalized patients with chronic leg ulcers and positive P. aeruginosa cultures.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 87 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 14 16%
Student > Ph. D. Student 13 15%
Student > Bachelor 11 13%
Researcher 7 8%
Student > Postgraduate 4 5%
Other 7 8%
Unknown 31 36%
Readers by discipline Count As %
Immunology and Microbiology 12 14%
Medicine and Dentistry 10 11%
Biochemistry, Genetics and Molecular Biology 9 10%
Agricultural and Biological Sciences 9 10%
Nursing and Health Professions 5 6%
Other 10 11%
Unknown 32 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2017.
All research outputs
#15,267,106
of 25,522,520 outputs
Outputs from BMC Infectious Diseases
#3,956
of 8,646 outputs
Outputs of similar age
#157,024
of 314,186 outputs
Outputs of similar age from BMC Infectious Diseases
#61
of 112 outputs
Altmetric has tracked 25,522,520 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,646 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.7. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,186 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 112 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.