Title |
Molecular xenomonitoring of Dirofilaria immitis and Dirofilaria repens in mosquitoes from north-eastern Italy by real-time PCR coupled with melting curve analysis
|
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Published in |
Parasites & Vectors, April 2012
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DOI | 10.1186/1756-3305-5-76 |
Pubmed ID | |
Authors |
Maria Stefania Latrofa, Fabrizio Montarsi, Silvia Ciocchetta, Giada Annoscia, Filipe Dantas-Torres, Silvia Ravagnan, Gioia Capelli, Domenico Otranto |
Abstract |
Dirofilaria immitis and Dirofilaria repens are transmitted by bloodsucking culicid mosquitoes belonging to Culex, Aedes, Ochlerotatus, Anopheles and Mansonia genera. The detection of filarioids in mosquitoes for assessing distribution of vectors and/or of pathogens in a given area (also known as "xenomonitoring"), when based on individual dissection of wild-caught female mosquitoes is time consuming and hardly applicable in large epidemiological surveys. Our study aimed to evaluate the recently developed duplex real-time PCR for screening large number of culicids and to assess their positivity for D. immitis and D. repens in an area where both species are endemic. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Ukraine | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Indonesia | 1 | 1% |
Malaysia | 1 | 1% |
United Kingdom | 1 | 1% |
France | 1 | 1% |
Unknown | 65 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 19 | 28% |
Professor > Associate Professor | 8 | 12% |
Student > Bachelor | 8 | 12% |
Student > Ph. D. Student | 7 | 10% |
Student > Master | 7 | 10% |
Other | 9 | 13% |
Unknown | 11 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 35% |
Veterinary Science and Veterinary Medicine | 12 | 17% |
Biochemistry, Genetics and Molecular Biology | 10 | 14% |
Medicine and Dentistry | 3 | 4% |
Immunology and Microbiology | 2 | 3% |
Other | 6 | 9% |
Unknown | 12 | 17% |