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Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity

Overview of attention for article published in Plant Methods, February 2020
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Title
Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity
Published in
Plant Methods, February 2020
DOI 10.1186/s13007-020-00568-7
Pubmed ID
Authors

Guo Wen, Jiangbo Dang, Zhongyi Xie, Jinying Wang, Pengfei Jiang, Qigao Guo, Guolu Liang

Abstract

Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants. Based on this imbalance in chromosome dosage, a new approach (referred to as 'SSR-qPCR') combining simple sequence repeat (SSR) markers and quantitative real-time PCR (qPCR) has been developed and utilized to detect some common aneuploids irrespective of heterozygosity. We screened 17 specific SSR markers covering all loquat linkage groups and redesigned 6 pairs of primers for SSR markers that can detect loquat chromosome aneuploidies. The SSR-qPCR detection results obtained for hybrid progeny and open-pollination progeny of triploid loquat showed diagnostic accuracies of 88.9% and 62.5%, respectively, compared with the chromosome preparation results. SSR-qPCR can detect loquat aneuploids and be used to construct the entire molecular karyotypes of aneuploid individuals. Therefore, this method offers a novel alternative for the detection of chromosome aneuploidies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 14%
Unknown 6 86%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 14%
Unknown 6 86%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2020.
All research outputs
#15,601,089
of 23,195,584 outputs
Outputs from Plant Methods
#841
of 1,096 outputs
Outputs of similar age
#223,037
of 361,242 outputs
Outputs of similar age from Plant Methods
#31
of 40 outputs
Altmetric has tracked 23,195,584 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,096 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
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