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Re-partitioning of Cu and Zn isotopes by modified protein expression

Overview of attention for article published in Geochemical Transactions, October 2008
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Title
Re-partitioning of Cu and Zn isotopes by modified protein expression
Published in
Geochemical Transactions, October 2008
DOI 10.1186/1467-4866-9-11
Pubmed ID
Authors

Anette Büchl, Chris J Hawkesworth, K Vala Ragnarsdottir, David R Brown

Abstract

Cu and Zn have naturally occurring non radioactive isotopes, and their isotopic systematics in a biological context are poorly understood. In this study we used double focussing mass spectroscopy to determine the ratios for these isotopes for the first time in mouse brain. The Cu and Zn isotope ratios for four strains of wild-type mice showed no significant difference (delta 65Cu -0.12 to -0.78 permil; delta 66Zn -0.23 to -0.48 permil). We also looked at how altering the expression of a single copper binding protein, the prion protein (PrP), alters the isotope ratios. Both knockout and overexpression of PrP had no significant effect on the ratio of Cu isotopes. Mice brains expressing mutant PrP lacking the known metal binding domain have delta 65Cu isotope values of on average 0.57 permil higher than wild-type mouse brains. This implies that loss of the copper binding domain of PrP increases the level of 65Cu in the brain. delta 66Zn isotope values of the transgenic mouse brains are enriched for 66Zn to the wild-type mouse brains. Here we show for the first time that the expression of a single protein can alter the partitioning of metal isotopes in mouse brains. The results imply that the expression of the prion protein can alter cellular Cu isotope content.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 19%
Professor 4 15%
Student > Master 4 15%
Student > Ph. D. Student 4 15%
Other 2 8%
Other 4 15%
Unknown 3 12%
Readers by discipline Count As %
Chemistry 4 15%
Earth and Planetary Sciences 4 15%
Biochemistry, Genetics and Molecular Biology 4 15%
Agricultural and Biological Sciences 4 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 4 15%
Unknown 5 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 May 2012.
All research outputs
#3,111,452
of 4,505,992 outputs
Outputs from Geochemical Transactions
#25
of 38 outputs
Outputs of similar age
#48,618
of 75,676 outputs
Outputs of similar age from Geochemical Transactions
#2
of 4 outputs
Altmetric has tracked 4,505,992 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 38 research outputs from this source. They receive a mean Attention Score of 2.2. This one scored the same or higher as 13 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 75,676 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.