Title |
Crude subcellular fractionation of cultured mammalian cell lines
|
---|---|
Published in |
BMC Research Notes, December 2009
|
DOI | 10.1186/1756-0500-2-243 |
Pubmed ID | |
Authors |
Paul Holden, William A Horton |
Abstract |
The expression and study of recombinant proteins in mammalian culture systems can be complicated during the cell lysis procedure by contaminating proteins from cellular compartments distinct from those within which the protein of interest resides and also by solubility issues that may arise from the use of a single lysis buffer. Partial subcellular fractionation using buffers of increasing stringency, rather than whole cell lysis is one way in which to avoid or reduce this contamination and ensure complete recovery of the target protein. Currently published protocols involve time consuming centrifugation steps which may require expensive equipment and commercially available kits can be prohibitively expensive when handling large or multiple samples. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 8 | 1% |
Germany | 5 | <1% |
United Kingdom | 4 | <1% |
South Africa | 2 | <1% |
Italy | 1 | <1% |
Brazil | 1 | <1% |
India | 1 | <1% |
Portugal | 1 | <1% |
Austria | 1 | <1% |
Other | 3 | <1% |
Unknown | 507 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 149 | 28% |
Researcher | 126 | 24% |
Student > Master | 52 | 10% |
Student > Bachelor | 44 | 8% |
Student > Doctoral Student | 33 | 6% |
Other | 67 | 13% |
Unknown | 63 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 201 | 38% |
Biochemistry, Genetics and Molecular Biology | 156 | 29% |
Medicine and Dentistry | 26 | 5% |
Chemistry | 23 | 4% |
Immunology and Microbiology | 19 | 4% |
Other | 26 | 5% |
Unknown | 83 | 16% |