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Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro

Overview of attention for article published in Journal of Translational Medicine, January 2009
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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blogs
1 blog
twitter
1 X user
facebook
1 Facebook page
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1 Google+ user

Citations

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11 Dimensions

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43 Mendeley
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Title
Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro
Published in
Journal of Translational Medicine, January 2009
DOI 10.1186/1479-5876-7-6
Pubmed ID
Authors

Tatiana Jazedje, Mariane Secco, Natássia M Vieira, Eder Zucconi, Thomaz R Gollop, Mariz Vainzof, Mayana Zatz

Abstract

The dystrophin gene, located at Xp21, codifies dystrophin, which is part of a protein complex responsible for the membrane stability of muscle cells. Its absence on muscle causes Duchenne Muscular Dystrophy (DMD), a severe disorder, while a defect of muscle dystrophin causes Becker Muscular Dystrophy (DMB), a milder disease. The replacement of the defective muscle through stem cells transplantation is a possible future treatment for these patients. Our objective was to analyze the potential of CD34+ stem cells from umbilical cord blood to differentiate in muscle cells and express dystrophin, in vitro. Protein expression was analyzed by Immunofluorescence, Western Blotting (WB) and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). CD34+ stem cells and myoblasts from a DMD affected patient started to fuse with muscle cells immediately after co-cultures establishment. Differentiation in mature myotubes was observed after 15 days and dystrophin-positive regions were detected through Immunofluorescence analysis. However, WB or RT-PCR analysis did not detect the presence of normal dystrophin in co-cultures of CD34+ and DMD or DMB affected patients' muscle cells. In contrast, some CD34+ stem cells differentiated in dystrophin producers' muscle cells, what was observed by WB, reinforcing that this progenitor cell has the potential to originate muscle dystrophin in vitro, and not just in vivo like reported before.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 5%
Portugal 1 2%
Poland 1 2%
Brazil 1 2%
Unknown 38 88%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 16%
Student > Ph. D. Student 6 14%
Researcher 6 14%
Student > Master 6 14%
Other 5 12%
Other 10 23%
Unknown 3 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 51%
Biochemistry, Genetics and Molecular Biology 6 14%
Medicine and Dentistry 5 12%
Computer Science 1 2%
Social Sciences 1 2%
Other 3 7%
Unknown 5 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2020.
All research outputs
#3,166,284
of 22,668,244 outputs
Outputs from Journal of Translational Medicine
#511
of 3,954 outputs
Outputs of similar age
#16,203
of 169,780 outputs
Outputs of similar age from Journal of Translational Medicine
#2
of 7 outputs
Altmetric has tracked 22,668,244 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,954 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.5. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 169,780 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.