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Mendeley readers
| Title |
Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos
|
|---|---|
| Published in |
BMC Biotechnology, June 2012
|
| DOI | 10.1186/1472-6750-12-32 |
| Pubmed ID | |
| Authors |
Okhyun Lee, Charles R Tyler, Tetsuhiro Kudoh |
| Abstract |
Oestrogenic contaminants are widespread in the aquatic environment and have been shown to induce adverse effects in both wildlife (most notably in fish) and humans, raising international concern. Available detecting and testing systems are limited in their capacity to elucidate oestrogen signalling pathways and physiological impacts. Here we developed a transient expression assay to investigate the effects of oestrogenic chemicals in fish early life stages and to identify target organs for oestrogenic effects. To enhance the response sensitivity to oestrogen, we adopted the use of multiple tandem oestrogen responsive elements (EREc38) in a Tol2 transposon mediated Gal4ff-UAS system. The plasmid constructed (pTol2_ERE-TATA-Gal4ff), contains three copies of oestrogen response elements (3ERE) that on exposure to oestrogen induces expression of Gal4ff which this in turn binds Gal4-responsive Upstream Activated Sequence (UAS) elements, driving the expression of a second reporter gene, EGFP (Enhanced Green Fluorescent Protein). |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 40 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 26% |
| Student > Bachelor | 7 | 17% |
| Researcher | 5 | 12% |
| Student > Ph. D. Student | 5 | 12% |
| Other | 2 | 5% |
| Other | 6 | 14% |
| Unknown | 6 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 33% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Environmental Science | 5 | 12% |
| Medicine and Dentistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 5 | 12% |
| Unknown | 8 | 19% |