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Mendeley readers
Attention Score in Context
| Title |
Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos
|
|---|---|
| Published in |
BMC Biotechnology, June 2012
|
| DOI | 10.1186/1472-6750-12-32 |
| Pubmed ID | |
| Authors |
Okhyun Lee, Charles R Tyler, Tetsuhiro Kudoh |
| Abstract |
Oestrogenic contaminants are widespread in the aquatic environment and have been shown to induce adverse effects in both wildlife (most notably in fish) and humans, raising international concern. Available detecting and testing systems are limited in their capacity to elucidate oestrogen signalling pathways and physiological impacts. Here we developed a transient expression assay to investigate the effects of oestrogenic chemicals in fish early life stages and to identify target organs for oestrogenic effects. To enhance the response sensitivity to oestrogen, we adopted the use of multiple tandem oestrogen responsive elements (EREc38) in a Tol2 transposon mediated Gal4ff-UAS system. The plasmid constructed (pTol2_ERE-TATA-Gal4ff), contains three copies of oestrogen response elements (3ERE) that on exposure to oestrogen induces expression of Gal4ff which this in turn binds Gal4-responsive Upstream Activated Sequence (UAS) elements, driving the expression of a second reporter gene, EGFP (Enhanced Green Fluorescent Protein). |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 39 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 27% |
| Student > Bachelor | 7 | 17% |
| Researcher | 5 | 12% |
| Student > Ph. D. Student | 5 | 12% |
| Other | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 6 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 34% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Environmental Science | 5 | 12% |
| Medicine and Dentistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 4 | 10% |
| Unknown | 8 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 January 2024.
All research outputs
#8,430,276
of 25,177,382 outputs
Outputs from BMC Biotechnology
#458
of 973 outputs
Outputs of similar age
#57,785
of 170,089 outputs
Outputs of similar age from BMC Biotechnology
#7
of 13 outputs
Altmetric has tracked 25,177,382 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 973 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,089 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.