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Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

Overview of attention for article published in BMC Microbiology, June 2012
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Title
Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil
Published in
BMC Microbiology, June 2012
DOI 10.1186/1471-2180-12-93
Pubmed ID
Authors

Mirco Iotti, Marco Leonardi, Marilena Oddis, Elena Salerni, Elena Baraldi, Alessandra Zambonelli

Abstract

Tuber magnatum, the Italian white truffle, is the most sought-after edible ectomycorrhizal mushroom. Previous studies report the difficulties of detecting its mycorrhizas and the widespread presence of its mycelium in natural production areas, suggesting that the soil mycelium could be a good indicator to evaluate its presence in the soil. In this study a specific real-time PCR assay using TaqMan chemistry was developed to detect and quantify T. magnatum in soil. This technique was then applied to four natural T. magnatum truffières located in different regions of Italy to validate the method under different environmental conditions.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 1%
Chile 1 1%
Italy 1 1%
Spain 1 1%
Greece 1 1%
United States 1 1%
Algeria 1 1%
Unknown 64 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 22 31%
Student > Ph. D. Student 12 17%
Student > Bachelor 7 10%
Professor 4 6%
Student > Master 4 6%
Other 9 13%
Unknown 13 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 43 61%
Environmental Science 6 8%
Biochemistry, Genetics and Molecular Biology 4 6%
Computer Science 1 1%
Medicine and Dentistry 1 1%
Other 0 0%
Unknown 16 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 June 2012.
All research outputs
#15,246,403
of 22,669,724 outputs
Outputs from BMC Microbiology
#1,750
of 3,162 outputs
Outputs of similar age
#106,382
of 166,899 outputs
Outputs of similar age from BMC Microbiology
#18
of 26 outputs
Altmetric has tracked 22,669,724 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,162 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 166,899 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.