Title |
Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil
|
---|---|
Published in |
BMC Microbiology, June 2012
|
DOI | 10.1186/1471-2180-12-93 |
Pubmed ID | |
Authors |
Mirco Iotti, Marco Leonardi, Marilena Oddis, Elena Salerni, Elena Baraldi, Alessandra Zambonelli |
Abstract |
Tuber magnatum, the Italian white truffle, is the most sought-after edible ectomycorrhizal mushroom. Previous studies report the difficulties of detecting its mycorrhizas and the widespread presence of its mycelium in natural production areas, suggesting that the soil mycelium could be a good indicator to evaluate its presence in the soil. In this study a specific real-time PCR assay using TaqMan chemistry was developed to detect and quantify T. magnatum in soil. This technique was then applied to four natural T. magnatum truffières located in different regions of Italy to validate the method under different environmental conditions. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Italy | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 1% |
Chile | 1 | 1% |
Italy | 1 | 1% |
Spain | 1 | 1% |
Greece | 1 | 1% |
United States | 1 | 1% |
Algeria | 1 | 1% |
Unknown | 64 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 31% |
Student > Ph. D. Student | 12 | 17% |
Student > Bachelor | 7 | 10% |
Professor | 4 | 6% |
Student > Master | 4 | 6% |
Other | 9 | 13% |
Unknown | 13 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 43 | 61% |
Environmental Science | 6 | 8% |
Biochemistry, Genetics and Molecular Biology | 4 | 6% |
Computer Science | 1 | 1% |
Medicine and Dentistry | 1 | 1% |
Other | 0 | 0% |
Unknown | 16 | 23% |