Title |
A PCR assay detects a male-specific duplicated copy of Anti-Müllerian hormone (amh) in the lingcod (Ophiodon elongatus)
|
---|---|
Published in |
BMC Research Notes, April 2016
|
DOI | 10.1186/s13104-016-2030-6 |
Pubmed ID | |
Authors |
Eric B. Rondeau, Cassandra V. Laurie, Stewart C. Johnson, Ben F. Koop |
Abstract |
Anti-Müllerian hormone (amh) or Müllerian-inhibiting substance (mis) is a member of the transforming growth factor-β family of hormones. This gene plays a key role in vertebrate male sex-determination by inhibiting the development of the Müllerian ducts, and has been shown to be the master sex-determinant in the Patagonian pejerrey. In the lingcod, Ophiodon elongatus, both males and females share one copy of amh, however we have identified a second duplicate copy that appears solely in the male individuals. We have developed a PCR-based assay targeting the TGF-β domain of amh that provides a simple method with which to sex lingcod from a small amount of tissue. An analysis across 57 individuals gave a 100 % success rate in identifying the phenotypic sex. We present a simple method to sex lingcod through non-lethal tissue sampling. A third, independent, male-specific duplication of amh in a teleost fish has been identified in the lingcod. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 67% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 40% |
Student > Ph. D. Student | 6 | 30% |
Student > Bachelor | 2 | 10% |
Professor | 1 | 5% |
Student > Master | 1 | 5% |
Other | 1 | 5% |
Unknown | 1 | 5% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 30% |
Agricultural and Biological Sciences | 6 | 30% |
Environmental Science | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Mathematics | 1 | 5% |
Other | 2 | 10% |
Unknown | 3 | 15% |