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Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Overview of attention for article published in BMC Biotechnology, June 2012
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Title
Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species
Published in
BMC Biotechnology, June 2012
DOI 10.1186/1472-6750-12-33
Pubmed ID
Authors

Doreen Schwochow, Laurel EK Serieys, Robert K Wayne, Olaf Thalmann

Abstract

Since the emergence of next generation sequencing platforms, unprecedented opportunities have arisen in the study of natural vertebrate populations. In particular, insights into the genetic and epigenetic mechanisms of adaptation can be revealed through study of the expression profiles of genes. However, as a pre-requisite to expression profiling, care must be taken in RNA preparation as factors like DNA contamination, RNA integrity or transcript abundance can affect downstream applications. Here, we evaluated five commonly used RNA extraction methods using whole blood sampled under varying conditions from 20 wild carnivores.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 183 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Malaysia 1 <1%
Chile 1 <1%
India 1 <1%
Brazil 1 <1%
Spain 1 <1%
Denmark 1 <1%
Unknown 175 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 41 22%
Researcher 38 21%
Student > Master 21 11%
Student > Bachelor 20 11%
Student > Postgraduate 10 5%
Other 34 19%
Unknown 19 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 79 43%
Biochemistry, Genetics and Molecular Biology 28 15%
Medicine and Dentistry 12 7%
Veterinary Science and Veterinary Medicine 7 4%
Immunology and Microbiology 5 3%
Other 21 11%
Unknown 31 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 June 2012.
All research outputs
#17,660,193
of 22,669,724 outputs
Outputs from BMC Biotechnology
#728
of 934 outputs
Outputs of similar age
#121,867
of 164,426 outputs
Outputs of similar age from BMC Biotechnology
#12
of 13 outputs
Altmetric has tracked 22,669,724 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 934 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,426 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.