Title |
Immunoliposome-PCR: a generic ultrasensitive quantitative antigen detection system
|
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Published in |
Journal of Nanobiotechnology, June 2012
|
DOI | 10.1186/1477-3155-10-26 |
Pubmed ID | |
Authors |
Junkun He, David L Evers, Timothy J O’Leary, Jeffrey T Mason |
Abstract |
The accurate quantification of antigens at low concentrations over a wide dynamic range is needed for identifying biomarkers associated with disease and detecting protein interactions in high-throughput microarrays used in proteomics. Here we report the development of an ultrasensitive quantitative assay format called immunoliposome polymerase chain reaction (ILPCR) that fulfills these requirements. This method uses a liposome, with reporter DNA encapsulated inside and biotin-labeled polyethylene glycol (PEG) phospholipid conjugates incorporated into the outer surface of the liposome, as a detection reagent. The antigenic target is immobilized in the well of a microplate by a capture antibody and the liposome detection reagent is then coupled to a biotin-labeled second antibody through a NeutrAvidin bridge. The liposome is ruptured to release the reporter DNA, which serves as a surrogate to quantify the protein target using real-time PCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 2% |
Unknown | 55 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 30% |
Researcher | 11 | 20% |
Student > Master | 5 | 9% |
Student > Bachelor | 5 | 9% |
Professor > Associate Professor | 3 | 5% |
Other | 8 | 14% |
Unknown | 7 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 29% |
Biochemistry, Genetics and Molecular Biology | 8 | 14% |
Medicine and Dentistry | 5 | 9% |
Engineering | 5 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
Other | 9 | 16% |
Unknown | 9 | 16% |