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Quantitative and qualitative characterization of Two PD-L1 clones: SP263 and E1L3N

Overview of attention for article published in Diagnostic Pathology, May 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#18 of 992)
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

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12 tweeters
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2 patents

Citations

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56 Dimensions

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57 Mendeley
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Title
Quantitative and qualitative characterization of Two PD-L1 clones: SP263 and E1L3N
Published in
Diagnostic Pathology, May 2016
DOI 10.1186/s13000-016-0494-2
Pubmed ID
Authors

Jacquelyn Smith, Mark D. Robida, Krista Acosta, Bharathi Vennapusa, Amita Mistry, Greg Martin, Alton Yates, H. James Hnatyszyn

Abstract

Programmed Death Ligand 1 (PD-L1) is an immune modulating protein expressed on the surface of various inflammatory cells, including T Cells, B Cells, dendritic cells, and macrophages. PD-L1 represents an important diagnostic target; expression of PD-L1 on the surface of tumor cells, or within tumor-associated immune cells, is an important predictor of likely response to targeted therapies. In this study, we describe the optimization of immunohistochemistry (IHC) assays using two PD-L1 antibodies (SP263 and E1L3N) and compare the performance of the optimized assays. Fully automated immunohistochemical assays were optimized for the VENTANA PD-L1 (SP263) Rabbit Monoclonal Antibody and the PD-L1 (E1L3N®) XP® Rabbit mAb using instruments and detection chemistries from Ventana Medical Systems, Inc. ("SP263 assay" and "E1L3N assay," respectively). Tissue microarrays (TMAs) containing formalin fixed paraffin embedded (FFPE) non-small cell lung cancer (NSCLC) cases were used for the optimization and comparison staining. H scores were used for membrane scoring whereas percent positivity was used for tumor-associated immune cell scoring. One-hundred NSCLC TMA case cores each stained with the SP263 and E1L3N assays were evaluated by two pathologists in a blinded study. Analysis of these specimens showed that the SP263 assay was more sensitive and had a wider dynamic range than the E1L3N assay. For sensitivity, many cases were found to be negative for membrane staining with the E1L3N assay, but had measurable staining with the SP263 assay. Dynamic range was demonstrated by the SP263 assay having well-distributed H scores while the E1L3N assay had a significantly higher proportion of cases clustered in the lowest H score bins. For tumor-associated immune cell staining, the two assays identified similar amounts of cells staining in each case, but the SP263 assay gave overall darker staining. Since PD-L1 status is important for targeted therapies, having a specific and accurate diagnostic test is crucial for identifying patients who could benefit from these treatments. Due to its staining intensity, scoring range, and pathologist preference, the SP263 IHC assay has been deemed superior to the E1L3N IHC assay. Future clinical utility remains to be determined.

Twitter Demographics

The data shown below were collected from the profiles of 12 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 57 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 26%
Student > Master 12 21%
Student > Postgraduate 6 11%
Student > Ph. D. Student 3 5%
Student > Bachelor 2 4%
Other 7 12%
Unknown 12 21%
Readers by discipline Count As %
Medicine and Dentistry 21 37%
Biochemistry, Genetics and Molecular Biology 8 14%
Agricultural and Biological Sciences 4 7%
Nursing and Health Professions 2 4%
Engineering 2 4%
Other 5 9%
Unknown 15 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 August 2020.
All research outputs
#1,689,409
of 18,639,770 outputs
Outputs from Diagnostic Pathology
#18
of 992 outputs
Outputs of similar age
#32,751
of 275,166 outputs
Outputs of similar age from Diagnostic Pathology
#1
of 6 outputs
Altmetric has tracked 18,639,770 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 992 research outputs from this source. They receive a mean Attention Score of 2.5. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,166 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them