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A novel multi-epitope recombined protein for diagnosis of human brucellosis

Overview of attention for article published in BMC Infectious Diseases, May 2016
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Title
A novel multi-epitope recombined protein for diagnosis of human brucellosis
Published in
BMC Infectious Diseases, May 2016
DOI 10.1186/s12879-016-1552-9
Pubmed ID
Authors

Dehui Yin, Li Li, Xiuling Song, Han Li, Juan Wang, Wen Ju, Xiaofeng Qu, Dandan Song, Yushen Liu, Xiangjun Meng, Hongqian Cao, Weiyi Song, Rizeng Meng, Jinhua Liu, Juan Li, Kun Xu

Abstract

In epidemic regions of the world, brucellosis is a reemerging zoonosis with minimal mortality but is a serious public hygiene problem. Currently, there are various methods for brucellosis diagnosis, however few of them are available to be used to diagnose, especially for serious cross-reaction with other bacteria. To overcome this disadvantage, we explored a novel multi-epitope recombinant protein as human brucellosis diagnostic antigen. We established an indirect enzyme-linked immunosorbent assay (ELISA) based on this recombinant protein. 248 sera obtained from three different groups including patients with brucellosis (146 samples), non-brucellosis patients (82 samples), and healthy individuals (20 samples) were tested by indirect ELISA. To evaluate the assay, a receiver-operating characteristic (ROC) analysis and immunoblotting were carried out using these characterized serum samples. For this test, the area under the ROC curve was 0.9409 (95 % confidence interval, 0.9108 to 0.9709), and a sensitivity of 88.89 % and a specificity of 85.54 % was given with a cutoff value of 0.3865 from this ROC analysis. The Western blot results indicate that it is feasible to differentiate human brucellosis and non-brucellosis with the newly established method based on this recombinant protein. Our results obtained high diagnostic accuracy of the ELISA assay which encourage the use of this novel recombinant protein as diagnostic antigen to implement serological diagnosis of brucellosis.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Spain 1 1%
Unknown 65 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 13%
Researcher 9 13%
Student > Bachelor 9 13%
Student > Master 5 7%
Student > Postgraduate 4 6%
Other 10 15%
Unknown 21 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 16%
Agricultural and Biological Sciences 10 15%
Veterinary Science and Veterinary Medicine 6 9%
Immunology and Microbiology 6 9%
Medicine and Dentistry 5 7%
Other 4 6%
Unknown 25 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2016.
All research outputs
#20,330,976
of 22,875,477 outputs
Outputs from BMC Infectious Diseases
#6,480
of 7,689 outputs
Outputs of similar age
#286,195
of 333,164 outputs
Outputs of similar age from BMC Infectious Diseases
#130
of 155 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,689 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 155 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.