Title |
Taxonomer: an interactive metagenomics analysis portal for universal pathogen detection and host mRNA expression profiling
|
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Published in |
Genome Biology, May 2016
|
DOI | 10.1186/s13059-016-0969-1 |
Pubmed ID | |
Authors |
Steven Flygare, Keith Simmon, Chase Miller, Yi Qiao, Brett Kennedy, Tonya Di Sera, Erin H. Graf, Keith D. Tardif, Aurélie Kapusta, Shawn Rynearson, Chris Stockmann, Krista Queen, Suxiang Tong, Karl V. Voelkerding, Anne Blaschke, Carrie L. Byington, Seema Jain, Andrew Pavia, Krow Ampofo, Karen Eilbeck, Gabor Marth, Mark Yandell, Robert Schlaberg |
Abstract |
High-throughput sequencing enables unbiased profiling of microbial communities, universal pathogen detection, and host response to infectious diseases. However, computation times and algorithmic inaccuracies have hindered adoption. We present Taxonomer, an ultrafast, web-tool for comprehensive metagenomics data analysis and interactive results visualization. Taxonomer is unique in providing integrated nucleotide and protein-based classification and simultaneous host messenger RNA (mRNA) transcript profiling. Using real-world case-studies, we show that Taxonomer detects previously unrecognized infections and reveals antiviral host mRNA expression profiles. To facilitate data-sharing across geographic distances in outbreak settings, Taxonomer is publicly available through a web-based user interface. Taxonomer enables rapid, accurate, and interactive analyses of metagenomics data on personal computers and mobile devices. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 25 | 26% |
United Kingdom | 6 | 6% |
Australia | 6 | 6% |
France | 6 | 6% |
Germany | 2 | 2% |
Mexico | 2 | 2% |
Canada | 2 | 2% |
Finland | 1 | 1% |
Ireland | 1 | 1% |
Other | 10 | 10% |
Unknown | 35 | 36% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 51 | 53% |
Scientists | 41 | 43% |
Practitioners (doctors, other healthcare professionals) | 2 | 2% |
Science communicators (journalists, bloggers, editors) | 2 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | 2% |
Brazil | 4 | 1% |
United Kingdom | 3 | <1% |
Germany | 2 | <1% |
Sweden | 1 | <1% |
South Africa | 1 | <1% |
Canada | 1 | <1% |
Taiwan | 1 | <1% |
India | 1 | <1% |
Other | 4 | 1% |
Unknown | 293 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 75 | 24% |
Student > Ph. D. Student | 65 | 20% |
Student > Master | 45 | 14% |
Student > Bachelor | 35 | 11% |
Other | 15 | 5% |
Other | 44 | 14% |
Unknown | 39 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 104 | 33% |
Biochemistry, Genetics and Molecular Biology | 71 | 22% |
Computer Science | 27 | 8% |
Immunology and Microbiology | 20 | 6% |
Medicine and Dentistry | 17 | 5% |
Other | 32 | 10% |
Unknown | 47 | 15% |