@IgorUlitsky Indeed, this is also our experience. FOS/JUN are also known to have expression affected by cell preparation protocols https://t.co/mbL3gG1gwZ. So, ATAC might be only capturing a unwanted biological signal.
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@Singleron_Bio These look interesting. Are the gene expression datasets comparing cells after different lengths of storage and using this mastermix vs other reagents? We'd be keen to trial similar to what we did here: https://t.co/HDzTPLYKx8
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@LeoMachadodo @TrendsCellBio @FRelaix @PhiliMour @Sc_Ninjas @LGMartelotto @RelaixLab @SingleCellMedia @scell_papers Great but in our hands the cold dissociation has the lowest (if any) effect on the transcriptome. Methanol fixation had major issue of ambie
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RT @Al__Forrest: Pleased to announce this work is now published in @GenomeBiology https://t.co/sg7EQGJIkT Thanks to @ElenaDenisenko9 @Beli…