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Single-cell whole genome sequencing reveals no evidence for common aneuploidy in normal and Alzheimer’s disease neurons

Overview of attention for article published in Genome Biology, May 2016
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (94th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

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2 news outlets
blogs
2 blogs
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13 X users
facebook
1 Facebook page
wikipedia
1 Wikipedia page

Citations

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119 Dimensions

Readers on

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194 Mendeley
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Title
Single-cell whole genome sequencing reveals no evidence for common aneuploidy in normal and Alzheimer’s disease neurons
Published in
Genome Biology, May 2016
DOI 10.1186/s13059-016-0976-2
Pubmed ID
Authors

Hilda van den Bos, Diana C. J. Spierings, Aaron Taudt, Bjorn Bakker, David Porubský, Ester Falconer, Carolina Novoa, Nancy Halsema, Hinke G. Kazemier, Karina Hoekstra-Wakker, Victor Guryev, Wilfred F. A. den Dunnen, Floris Foijer, Maria Colomé-Tatché, Hendrikus W. G. M. Boddeke, Peter M. Lansdorp

Abstract

Alzheimer's disease (AD) is a neurodegenerative disease of the brain and the most common form of dementia in the elderly. Aneuploidy, a state in which cells have an abnormal number of chromosomes, has been proposed to play a role in neurodegeneration in AD patients. Several studies using fluorescence in situ hybridization have shown that the brains of AD patients contain an increased number of aneuploid cells. However, because the reported rate of aneuploidy in neurons ranges widely, a more sensitive method is needed to establish a possible role of aneuploidy in AD pathology. In the current study, we used a novel single-cell whole genome sequencing (scWGS) approach to assess aneuploidy in isolated neurons from the frontal cortex of normal control individuals (n = 6) and patients with AD (n = 10). The sensitivity and specificity of our method was shown by the presence of three copies of chromosome 21 in all analyzed neuronal nuclei of a Down's syndrome sample (n = 36). Very low levels of aneuploidy were found in the brains from control individuals (n = 589) and AD patients (n = 893). In contrast to other studies, we observe no selective gain of chromosomes 17 or 21 in neurons of AD patients. scWGS showed no evidence for common aneuploidy in normal and AD neurons. Therefore, our results do not support an important role for aneuploidy in neuronal cells in the pathogenesis of AD. This will need to be confirmed by future studies in larger cohorts.

X Demographics

X Demographics

The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 194 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 <1%
Netherlands 1 <1%
United Kingdom 1 <1%
Ukraine 1 <1%
United States 1 <1%
Unknown 189 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 57 29%
Researcher 27 14%
Student > Bachelor 22 11%
Student > Master 18 9%
Student > Doctoral Student 13 7%
Other 35 18%
Unknown 22 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 63 32%
Agricultural and Biological Sciences 47 24%
Neuroscience 19 10%
Medicine and Dentistry 13 7%
Computer Science 8 4%
Other 13 7%
Unknown 31 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2019.
All research outputs
#1,075,205
of 25,371,288 outputs
Outputs from Genome Biology
#775
of 4,467 outputs
Outputs of similar age
#19,980
of 353,666 outputs
Outputs of similar age from Genome Biology
#21
of 83 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,666 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 83 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.