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Exposure to common respiratory bacteria alters the airway epithelial response to subsequent viral infection

Overview of attention for article published in Respiratory Research, June 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

blogs
1 blog
twitter
6 X users
facebook
1 Facebook page

Citations

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48 Dimensions

Readers on

mendeley
141 Mendeley
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Title
Exposure to common respiratory bacteria alters the airway epithelial response to subsequent viral infection
Published in
Respiratory Research, June 2016
DOI 10.1186/s12931-016-0382-z
Pubmed ID
Authors

Carla Bellinghausen, Fahad Gulraiz, Alexandra C. A. Heinzmann, Mieke A. Dentener, Paul H. M. Savelkoul, Emiel F. Wouters, Gernot G. Rohde, Frank R. Stassen

Abstract

Colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as COPD or cystic fibrosis. Infections with respiratory viruses are known triggers of exacerbations of these diseases. We here investigated if pre-exposure to bacteria alters the response of lung epithelial cells to subsequent viral infection. Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae, Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates. Exposure of BEAS-2B cells to H. influenzae before and during RSV-infection synergistically increased the release of IL-6 (increase above calculated additive effect at 72 h: 56 % ± 3 %, mean ± SEM) and IL-8 (53 % ± 12 %). This effect was sustained even when bacteria were washed away before viral infection and was neither associated with enhanced viral replication, nor linked to increased expression of key pattern recognition receptors. P. aeruginosa enhanced the release of inflammatory cytokines to a similar extent, yet only if bacteria were also present during viral infection. S. pneumoniae did not enhance RSV-induced cytokine release. Surprisingly, adenovirus infection significantly reduced IL-6 release in cells exposed to either of the three tested bacterial strains by on average more than 50 %. Infection with influenza B on the other hand did not affect cytokine production in BEAS-2B cells exposed to the different bacterial strains. Pre-exposure of epithelial cells to bacteria alters the response to subsequent viral infection depending on the types of pathogen involved. These findings highlight the complexity of microbiome interactions in the airways, possibly contributing to the susceptibility to exacerbations and the natural course of airway diseases.

X Demographics

X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 141 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 <1%
Unknown 140 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 28 20%
Student > Ph. D. Student 27 19%
Student > Master 16 11%
Student > Bachelor 16 11%
Student > Doctoral Student 7 5%
Other 19 13%
Unknown 28 20%
Readers by discipline Count As %
Medicine and Dentistry 32 23%
Immunology and Microbiology 23 16%
Biochemistry, Genetics and Molecular Biology 21 15%
Agricultural and Biological Sciences 16 11%
Veterinary Science and Veterinary Medicine 4 3%
Other 10 7%
Unknown 35 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 October 2020.
All research outputs
#3,261,328
of 25,371,288 outputs
Outputs from Respiratory Research
#399
of 3,062 outputs
Outputs of similar age
#54,832
of 354,225 outputs
Outputs of similar age from Respiratory Research
#7
of 41 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,062 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 354,225 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.