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Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

Overview of attention for article published in Microbial Cell Factories, June 2016
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Title
Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine
Published in
Microbial Cell Factories, June 2016
DOI 10.1186/s12934-016-0494-7
Pubmed ID
Authors

Yang Li, Xujun Zhu, Xueyu Zhang, Jing Fu, Zhiwen Wang, Tao Chen, Xueming Zhao

Abstract

Genome streamlining has emerged as an effective strategy to boost the production efficiency of bio-based products. Many efforts have been made to construct desirable chassis cells by reducing the genome size of microbes. It has been reported that the genome-reduced Bacillus subtilis strain MBG874 showed clear advantages for the production of several heterologous enzymes including alkaline cellulase and protease. In addition to enzymes, B. subtilis is also used for the production of chemicals. To our best knowledge, it is still unknown whether genome reduction could be used to optimize the production of chemicals such as nucleoside products. In this study, we constructed a series of genome-reduced strains by deleting non-essential regions in the chromosome of B. subtilis 168. These strains with genome reductions ranging in size from 581.9 to 814.4 kb displayed markedly decreased growth rates, sporulation ratios, transformation efficiencies and maintenance coefficients, as well as increased cell yields. We re-engineered the genome-reduced strains to produce guanosine and thymidine, respectively. The strain BSK814G2, in which purA was knocked out, and prs, purF and guaB were co-overexpressed, produced 115.2 mg/L of guanosine, which was 4.4-fold higher compared to the control strain constructed by introducing the same gene modifications into the parental strain. We also constructed a thymidine producer by deleting the tdk gene and overexpressing the prs, ushA, thyA, dut, and ndk genes from Escherichia coli in strain BSK756, and the resulting strain BSK756T3 accumulated 151.2 mg/L thymidine, showing a 5.2-fold increase compared to the corresponding control strain. Genome-scale genetic manipulation has a variety of effects on the physiological characteristics and cell metabolism of B. subtilis. By introducing specific gene modifications related to guanosine and thymidine accumulation, respectively, we demonstrated that genome-reduced strains had greatly improved properties compared to the wild-type strain as chassis cells for the production of these two products. These strains also have great potential for the production of other nucleosides and similar derived chemicals.

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Geographical breakdown

Country Count As %
United Kingdom 1 2%
India 1 2%
Kazakhstan 1 2%
Canada 1 2%
Unknown 56 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 17%
Student > Master 10 17%
Researcher 9 15%
Student > Bachelor 5 8%
Other 3 5%
Other 9 15%
Unknown 14 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 31 52%
Agricultural and Biological Sciences 11 18%
Philosophy 1 2%
Nursing and Health Professions 1 2%
Immunology and Microbiology 1 2%
Other 2 3%
Unknown 13 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 June 2016.
All research outputs
#14,854,433
of 22,876,619 outputs
Outputs from Microbial Cell Factories
#928
of 1,604 outputs
Outputs of similar age
#201,742
of 339,345 outputs
Outputs of similar age from Microbial Cell Factories
#27
of 44 outputs
Altmetric has tracked 22,876,619 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,604 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,345 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.