Title |
Complete genome sequence of Streptococcus agalactiae strain GBS85147 serotype of type Ia isolated from human oropharynx
|
---|---|
Published in |
Environmental Microbiome, June 2016
|
DOI | 10.1186/s40793-016-0158-6 |
Pubmed ID | |
Authors |
Edgar Lacerda de Aguiar, Diego César Batista Mariano, Marcus Vinícius Canário Viana, Leandro de Jesus Benevides, Flávia de Souza Rocha, Letícia de Castro Oliveira, Felipe Luiz Pereira, Fernanda Alves Dorella, Carlos Augusto Gomes Leal, Alex Fiorini de Carvalho, Gabriela Silva Santos, Ana Luiza Mattos-Guaraldi, Prescilla Emy Nagao, Siomar de Castro Soares, Syed Shah Hassan, Anne Cybele Pinto, Henrique César Pereira Figueiredo, Vasco Azevedo |
Abstract |
Streptococcus agalactiae, also referred to as Group B Streptococcus, is a frequent resident of the rectovaginal tract in humans, and a major cause of neonatal infection. The pathogen can also infect adults with underlying disease, particularly the elderly and immunocompromised ones. In addition, S. agalactiae is a known fish pathogen, which compromises food safety and represents a zoonotic hazard. This study provides valuable structural, functional and evolutionary genomic information of a human S. agalactiae serotype Ia (ST-103) GBS85147 strain isolated from the oropharynx of an adult patient from Rio de Janeiro, thereby representing the first human isolate in Brazil. We used the Ion Torrent PGM platform with the 200 bp fragment library sequencing kit. The sequencing generated 578,082,183 bp, distributed among 2,973,022 reads, resulting in an approximately 246-fold mean coverage depth and was assembled using the Mira Assembler v3.9.18. The S. agalactiae strain GBS85147 comprises of a circular chromosome with a final genome length of 1,996,151 bp containing 1,915 protein-coding genes, 18 rRNA, 63 tRNA, 2 pseudogenes and a G + C content of 35.48 %. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 63 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 16% |
Student > Master | 9 | 14% |
Student > Ph. D. Student | 8 | 13% |
Student > Bachelor | 4 | 6% |
Student > Doctoral Student | 3 | 5% |
Other | 8 | 13% |
Unknown | 21 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 19% |
Agricultural and Biological Sciences | 10 | 16% |
Immunology and Microbiology | 6 | 10% |
Computer Science | 2 | 3% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Other | 10 | 16% |
Unknown | 21 | 33% |