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The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer

Overview of attention for article published in Journal of Experimental & Clinical Cancer Research, June 2016
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Title
The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer
Published in
Journal of Experimental & Clinical Cancer Research, June 2016
DOI 10.1186/s13046-016-0369-0
Pubmed ID
Authors

Jie Tang, Yi Zhu, Kunling Xie, Xiaoyu Zhang, Xiaofei Zhi, Weizhi Wang, Zheng Li, Qun Zhang, Linjun Wang, Jiwei Wang, Zekuan Xu

Abstract

MUC4 is a high molecular weight membrane protein that is overexpressed in pancreatic cancer (PC) and is associated with the development and progression of this disease. However, the exact mechanisms through which MUC4 domains promote these biological processes have rarely been studied, partly because of its high molecular weight, difficulty to overexpress it. Here, we use MUC4/Y, one of the MUC4 transcript variants, as a model molecule to investigate the AMOP-domain of MUC4(MUC/Y). We used cell proliferation, migration, invasion and tube formation assays in vitro to explore the abilities of AMOP domain in PC. In vivo, the matrigel plug assay, orthotopic implantation and Kaplan-Meier survival curves were used to check the results we observed in vitro. Finally, we discovered the underlying mechanism through western blot and immunofluorescence. We found that MUC4/Y overexpression could enhance the angiogenic and metastatic properties of PC cells, both in vitro and in vivo. However, the deletion of AMOP domain could cutback these phenomena. Additionally, Kaplan-Meier survival curves showed that mice injected with MUC4/Y overexpressed cells had shorter survival time, compared with empty-vector-transfected cells (MUC4/Y-EV), or cells expressing MUC4/Y without the AMOP domain (MUC4/Y-AMOP(△)). Our data also showed that overexpression of MUC4/Y could activate NOTCH3 signaling, increasing the expression of downstream genes: VEGF-A, MMP-9 and ANG-2. The AMOP domain had an important role in MUC4/Y (MUC4)-mediated tumour angiogenesis and metastasis of PC cells; and the NOTCH3 signaling was involved. These findings provided new insights into PC therapies. Our study also supplies a new method to study other high molecular membrane proteins.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 9%
Unknown 10 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Ph. D. Student 2 18%
Student > Bachelor 2 18%
Student > Master 2 18%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 27%
Medicine and Dentistry 2 18%
Agricultural and Biological Sciences 2 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Unknown 3 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2016.
All research outputs
#6,828,275
of 7,885,400 outputs
Outputs from Journal of Experimental & Clinical Cancer Research
#292
of 412 outputs
Outputs of similar age
#223,515
of 268,042 outputs
Outputs of similar age from Journal of Experimental & Clinical Cancer Research
#19
of 30 outputs
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So far Altmetric has tracked 412 research outputs from this source. They receive a mean Attention Score of 1.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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