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Genetic background affects induced pluripotent stem cell generation

Overview of attention for article published in Stem Cell Research & Therapy, August 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)

Mentioned by

blogs
1 blog
twitter
2 tweeters
wikipedia
1 Wikipedia page

Citations

dimensions_citation
21 Dimensions

Readers on

mendeley
52 Mendeley
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Title
Genetic background affects induced pluripotent stem cell generation
Published in
Stem Cell Research & Therapy, August 2012
DOI 10.1186/scrt121
Pubmed ID
Authors

Lauren V Schnabel, Christian M Abratte, John C Schimenti, Teresa L Southard, Lisa A Fortier

Abstract

ABSTRACT: INTRODUCTION: The influence of genetic background on the ability to generate induced pluripotent stem cells (iPSCs) has the potential to impact future applications, but has yet to be examined in detail. The purpose of this study was to determine if genetic background affects the efficiency of generating iPSCs during early reprograming as well as the pluripotent stability of the iPSCs during later stages of reprograming. METHODS: Mouse embryonic fibroblasts (MEFs) were isolated from six strains of mice (NON/LtJ; C57BL/6J; DBA/2J; BALB/cJ; 129S1/SvlmJ; CAST/EiJ) that were selected based on genetic diversity and differences in ability to produce embryonic stem cell (ESC) lines. MEFs were reprogramed via doxycycline-inducible lentiviral transduction of murine Oct4, Klf4, Sox2, and c-Myc. Differences in efficiency to generate iPSCs were assessed by comparing the total number of colonies, the percentage of colonies positive for alkaline phosphatase staining and the percentage of cells positive for SSEA1. iPSC colonies were expanded to establish doxycycline-independent cell lines whose pluripotency was then evaluated via ability to form teratomas in NOD.CB17-Prkdcscid/J mice. Proliferation of non-transduced parent MEFs from each strain was also examined over ten days under conditions that simulated reprograming. RESULTS: NON/LtJ and CAST/EiJ strains were more efficient than other strains in generating iPSCs for all parameters measured and parent MEFs from these strains were more proliferative than those from other strains. Doxycycline-independent iPSC lines were established using standard conditions for all strains except BALB/cJ, which required a higher concentration (5x) of leukemia inhibitory factor (LIF). iPSCs from all strains were capable of producing teratomas in NOD.CB17-Prkdcscid/J mice. CONCLUSIONS: The results of this study suggest that genetic background does affect iPSC generation and pluripotent stability. In addition, our results demonstrate that strain differences in efficiency to generate iPSCs during the early stages of reprograming are correlated with those observed in proliferation of parent MEFs. These findings have important implications both for future iPSC applications as well as for future investigation into determining the genes responsible for reprograming efficiency and stability.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Russia 1 2%
Unknown 50 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 35%
Researcher 8 15%
Student > Master 7 13%
Professor 5 10%
Student > Bachelor 2 4%
Other 9 17%
Unknown 3 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 40%
Biochemistry, Genetics and Molecular Biology 12 23%
Medicine and Dentistry 5 10%
Veterinary Science and Veterinary Medicine 4 8%
Engineering 2 4%
Other 5 10%
Unknown 3 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2014.
All research outputs
#1,876,835
of 17,351,915 outputs
Outputs from Stem Cell Research & Therapy
#129
of 1,743 outputs
Outputs of similar age
#13,714
of 133,926 outputs
Outputs of similar age from Stem Cell Research & Therapy
#1
of 4 outputs
Altmetric has tracked 17,351,915 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,743 research outputs from this source. They receive a mean Attention Score of 4.9. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 133,926 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them