Title |
Antiviral activity of micafungin against enterovirus 71
|
---|---|
Published in |
Virology Journal, June 2016
|
DOI | 10.1186/s12985-016-0557-8 |
Pubmed ID | |
Authors |
Chonsaeng Kim, Hyunju Kang, Dong-eun Kim, Jae-Hyoung Song, Miri Choi, Mingu Kang, Kyungjin Lee, Hae Soo Kim, Jin Soo Shin, Hyejeong Jeong, Sunhee Jung, Sang-Bae Han, Jong Heon Kim, Hyun-Jeong Ko, Chong-Kyo Lee, Meehyein Kim, Sungchan Cho |
Abstract |
Enterovirus 71 (EV71) is a major causative agent of hand-foot-mouth disease (HFMD) and also causes severe neurological complications, leading to fatality in young children. However, no effective therapy is currently available for the treatment of this infection. We identified small-molecule inhibitors of EV71 from a screen of 968 Food and Drug Administration (FDA)-approved drugs, with which clinical application for EV71-associated diseases would be more feasible, using EV71 subgenomic replicon system. Primary hits were extensively evaluated for their antiviral activities in EV71-infected cells. We identified micafungin, an echinocandin antifungal drug, as a novel inhibitor of EV71. Micafungin potently inhibits the proliferation of EV71 as well as the replication of EV71 replicon in cells with a low micromolar IC50 (~5 μM). The strong antiviral effect of micafungin on EV71 replicon and the result from time-of-addition experiment demonstrated a targeting of micafungin on virion-independent intracellular process(es) during EV71 infection. Moreover, an extensive analysis excluded the involvement of 2C and 3A proteins, IRES-dependent translation, and also that of polyprotein processing in the antiviral effect of micafungin. Our research revealed a new indication of micafungin as an effective inhibitor of EV71, which is the first case reporting antiviral activity of micafungin, an antifungal drug. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Turkey | 1 | 33% |
Japan | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 33% |
Scientists | 1 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 25% |
Researcher | 4 | 14% |
Student > Ph. D. Student | 3 | 11% |
Student > Doctoral Student | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 3 | 11% |
Unknown | 7 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 18% |
Medicine and Dentistry | 5 | 18% |
Chemistry | 4 | 14% |
Immunology and Microbiology | 3 | 11% |
Agricultural and Biological Sciences | 2 | 7% |
Other | 2 | 7% |
Unknown | 7 | 25% |