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Mendeley readers
| Title |
Nature of bacterial colonization influences transcription of mucin genes in mice during the first week of life
|
|---|---|
| Published in |
BMC Research Notes, August 2012
|
| DOI | 10.1186/1756-0500-5-402 |
| Pubmed ID | |
| Authors |
Anders Bergström, Matilde B Kristensen, Martin I Bahl, Stine B Metzdorff, Lisbeth N Fink, Hanne Frøkiær, Tine R Licht |
| Abstract |
Postnatal regulation of the small intestinal mucus layer is potentially important in the development of adult gut functionality. We hypothesized that the nature of bacterial colonization affects mucus gene regulation in early life.We thus analyzed the influence of the presence of a conventional microbiota as well as two selected monocolonizing bacterial strains on the transcription of murine genes involved in mucus layer development during the first week of life.Mouse pups (N = 8/group) from differently colonized dams: Germ-free (GF), conventional specific pathogen free (SPF), monocolonized with either Lactobacillus acidophilus NCFM (Lb) or Escherichia coli Nissle (Ec) were analyzed by qPCR on isolated ileal tissue sections from postnatal days 1 and 6 (PND1, PND6) after birth with respect to: (i) transcription of specific genes involved in mucus production (Muc1-4, Tff3) and (ii) amounts of 16S rRNA of Lactobacillus and E. coli. Quantification of 16S rRNA genes was performed to obtain a measure for amounts of colonized bacteria. |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Croatia | 1 | 1% |
| Germany | 1 | 1% |
| Unknown | 89 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 28 | 30% |
| Researcher | 16 | 17% |
| Student > Master | 15 | 16% |
| Student > Postgraduate | 7 | 8% |
| Student > Doctoral Student | 6 | 7% |
| Other | 10 | 11% |
| Unknown | 10 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 41% |
| Immunology and Microbiology | 14 | 15% |
| Biochemistry, Genetics and Molecular Biology | 13 | 14% |
| Medicine and Dentistry | 6 | 7% |
| Business, Management and Accounting | 1 | 1% |
| Other | 6 | 7% |
| Unknown | 14 | 15% |