Title |
A novel Rho-dependent pathway that drives interaction of fascin-1 with p-Lin-11/Isl-1/Mec-3 kinase (LIMK) 1/2 to promote fascin-1/actin binding and filopodia stability
|
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Published in |
BMC Biology, August 2012
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DOI | 10.1186/1741-7007-10-72 |
Pubmed ID | |
Authors |
Asier Jayo, Maddy Parsons, Josephine C Adams |
Abstract |
Fascin-1 is an actin crosslinking protein that is important for the assembly of cell protrusions in neurons, skeletal and smooth muscle, fibroblasts, and dendritic cells. Although absent from most normal adult epithelia, fascin-1 is upregulated in many human carcinomas, and is associated with poor prognosis because of its promotion of carcinoma cell migration, invasion, and metastasis. Rac and Cdc42 small guanine triphosphatases have been identified as upstream regulators of the association of fascin-1 with actin, but the possible role of Rho has remained obscure. Additionally, experiments have been hampered by the inability to measure the fascin-1/actin interaction directly in intact cells. We investigated the hypothesis that fascin-1 is a functional target of Rho in normal and carcinoma cells, using experimental approaches that included a novel fluorescence resonance energy transfer (FRET)/fluorescence lifetime imaging (FLIM) method to measure the interaction of fascin-1 with actin. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 40% |
France | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 40% |
Science communicators (journalists, bloggers, editors) | 1 | 20% |
Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 2% |
Singapore | 1 | 2% |
Korea, Republic of | 1 | 2% |
Japan | 1 | 2% |
United States | 1 | 2% |
Unknown | 56 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 16 | 26% |
Researcher | 12 | 20% |
Student > Master | 9 | 15% |
Student > Doctoral Student | 5 | 8% |
Student > Bachelor | 3 | 5% |
Other | 6 | 10% |
Unknown | 10 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 34% |
Biochemistry, Genetics and Molecular Biology | 14 | 23% |
Medicine and Dentistry | 6 | 10% |
Immunology and Microbiology | 3 | 5% |
Unspecified | 1 | 2% |
Other | 4 | 7% |
Unknown | 12 | 20% |