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Conditionally essential genes for survival during starvation in Enterococcus faecium E745

Overview of attention for article published in BMC Genomics, August 2020
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

twitter
20 tweeters

Citations

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3 Dimensions

Readers on

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9 Mendeley
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Title
Conditionally essential genes for survival during starvation in Enterococcus faecium E745
Published in
BMC Genomics, August 2020
DOI 10.1186/s12864-020-06984-2
Pubmed ID
Authors

Vincent de Maat, Sergio Arredondo-Alonso, Rob J. L. Willems, Willem van Schaik

Abstract

The nosocomial pathogen Enterococcus faecium can survive for prolonged periods of time on surfaces in the absence of nutrients. This trait is thought to contribute to the ability of E. faecium to spread among patients in hospitals. There is currently a lack of data on the mechanisms that are responsible for the ability of E. faecium to survive in the absence of nutrients. We performed a high-throughput transposon mutant library screening (Tn-seq) to identify genes that have a role in long-term survival during incubation in phosphate-buffered saline (PBS) at 20 °C. A total of 24 genes were identified by Tn-seq to contribute to survival in PBS, with functions associated with the general stress response, DNA repair, metabolism, and membrane homeostasis. The gene which was quantitatively most important for survival in PBS was usp (locus tag: EfmE745_02439), which is predicted to encode a 17.4 kDa universal stress protein. After generating a targeted deletion mutant in usp, we were able to confirm that usp significantly contributes to survival in PBS and this defect was restored by in trans complementation. The usp gene is present in 99% of a set of 1644 E. faecium genomes that collectively span the diversity of the species. We postulate that usp is a key determinant for the remarkable environmental robustness of E. faecium. Further mechanistic studies into usp and other genes identified in this study may shed further light on the mechanisms by which E. faecium can survive in the absence of nutrients for prolonged periods of time.

Twitter Demographics

The data shown below were collected from the profiles of 20 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 56%
Student > Ph. D. Student 2 22%
Student > Bachelor 1 11%
Student > Master 1 11%
Readers by discipline Count As %
Immunology and Microbiology 3 33%
Agricultural and Biological Sciences 3 33%
Biochemistry, Genetics and Molecular Biology 2 22%
Unknown 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 August 2020.
All research outputs
#2,273,491
of 18,908,107 outputs
Outputs from BMC Genomics
#898
of 9,656 outputs
Outputs of similar age
#59,651
of 308,593 outputs
Outputs of similar age from BMC Genomics
#2
of 6 outputs
Altmetric has tracked 18,908,107 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,656 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,593 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.