Title |
IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes
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Published in |
BMC Genomics, June 2012
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DOI | 10.1186/1471-2164-13-249 |
Pubmed ID | |
Authors |
Alejandro Reyes, Andrea Sandoval, Andrés Cubillos-Ruiz, Katherine E Varley, Ivan Hernández-Neuta, Sofía Samper, Carlos Martín, María Jesús García, Viviana Ritacco, Lucelly López, Jaime Robledo, María Mercedes Zambrano, Robi D Mitra, Patricia Del Portillo |
Abstract |
The insertion element IS6110 is one of the main sources of genomic variability in Mycobacterium tuberculosis, the etiological agent of human tuberculosis. Although IS 6110 has been used extensively as an epidemiological marker, the identification of the precise chromosomal insertion sites has been limited by technical challenges. Here, we present IS-seq, a novel method that combines high-throughput sequencing using Illumina technology with efficient combinatorial sample multiplexing to simultaneously probe 519 clinical isolates, identifying almost all the flanking regions of the element in a single experiment. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 3% |
Colombia | 2 | 3% |
Sweden | 1 | 2% |
Unknown | 59 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 21 | 33% |
Student > Ph. D. Student | 6 | 9% |
Professor | 5 | 8% |
Student > Master | 5 | 8% |
Student > Postgraduate | 4 | 6% |
Other | 10 | 16% |
Unknown | 13 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 13 | 20% |
Agricultural and Biological Sciences | 11 | 17% |
Biochemistry, Genetics and Molecular Biology | 9 | 14% |
Medicine and Dentistry | 8 | 13% |
Computer Science | 1 | 2% |
Other | 5 | 8% |
Unknown | 17 | 27% |