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IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes

Overview of attention for article published in BMC Genomics, June 2012
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Title
IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes
Published in
BMC Genomics, June 2012
DOI 10.1186/1471-2164-13-249
Pubmed ID
Authors

Alejandro Reyes, Andrea Sandoval, Andrés Cubillos-Ruiz, Katherine E Varley, Ivan Hernández-Neuta, Sofía Samper, Carlos Martín, María Jesús García, Viviana Ritacco, Lucelly López, Jaime Robledo, María Mercedes Zambrano, Robi D Mitra, Patricia Del Portillo

Abstract

The insertion element IS6110 is one of the main sources of genomic variability in Mycobacterium tuberculosis, the etiological agent of human tuberculosis. Although IS 6110 has been used extensively as an epidemiological marker, the identification of the precise chromosomal insertion sites has been limited by technical challenges. Here, we present IS-seq, a novel method that combines high-throughput sequencing using Illumina technology with efficient combinatorial sample multiplexing to simultaneously probe 519 clinical isolates, identifying almost all the flanking regions of the element in a single experiment.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Colombia 2 3%
Sweden 1 2%
Unknown 59 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 33%
Student > Ph. D. Student 6 9%
Professor 5 8%
Student > Master 5 8%
Student > Postgraduate 4 6%
Other 10 16%
Unknown 13 20%
Readers by discipline Count As %
Immunology and Microbiology 13 20%
Agricultural and Biological Sciences 11 17%
Biochemistry, Genetics and Molecular Biology 9 14%
Medicine and Dentistry 8 13%
Computer Science 1 2%
Other 5 8%
Unknown 17 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2013.
All research outputs
#20,165,369
of 22,675,759 outputs
Outputs from BMC Genomics
#9,240
of 10,614 outputs
Outputs of similar age
#150,069
of 166,060 outputs
Outputs of similar age from BMC Genomics
#91
of 102 outputs
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We're also able to compare this research output to 102 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.