Title |
Applications of CRISPR-Cas9 mediated genome engineering
|
---|---|
Published in |
Military Medical Research, May 2015
|
DOI | 10.1186/s40779-015-0038-1 |
Pubmed ID | |
Authors |
Xiao Yang |
Abstract |
Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development, normal physiology, and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9, is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models, somatic genome engineering, and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine, ranging from basic research to human gene therapy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 2 | 1% |
Portugal | 1 | <1% |
Netherlands | 1 | <1% |
Chile | 1 | <1% |
United Kingdom | 1 | <1% |
China | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Unknown | 186 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 44 | 23% |
Student > Ph. D. Student | 34 | 17% |
Student > Master | 26 | 13% |
Researcher | 24 | 12% |
Student > Doctoral Student | 6 | 3% |
Other | 23 | 12% |
Unknown | 38 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 63 | 32% |
Biochemistry, Genetics and Molecular Biology | 60 | 31% |
Medicine and Dentistry | 12 | 6% |
Immunology and Microbiology | 5 | 3% |
Neuroscience | 4 | 2% |
Other | 10 | 5% |
Unknown | 41 | 21% |