Title |
Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance
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Published in |
Journal of Translational Medicine, June 2016
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DOI | 10.1186/s12967-016-0940-7 |
Pubmed ID | |
Authors |
Mi Kyung Park, Young Ok Jung, Seon-Yeong Lee, Seung Hoon Lee, Yu Jung Heo, Eun Kyung Kim, Hye Jwa Oh, Young Mee Moon, Hye-Jin Son, Min Jung Park, Sung Hwan Park, Ho Youn Kim, Mi La Cho, Jun Ki Min |
Abstract |
Foxp3 is a key regulator of the development and function of regulatory T cells (Tregs), and its expression is thought to be T cell-restricted. We found that B cells in mice can express Foxp3 and B cells expressing Foxp3 may play a role in preventing the development of collagen-induced arthritis (CIA) in DBA/1J mice. Foxp3 expression was modulated in CD19(+) B cells by transfection with shRNA or using an over-expression construct. In addition, Foxp3-transfected B cells were adoptively transferred to CIA mice. We found that LPS or anti-IgM stimulation induced Foxp3 expression in B cells. Foxp3-expressing B cells were found in the spleens of mice. Over-expression of Foxp3 conferred a contact-dependent suppressive ability on proliferation of responder T cells. Down-regulation of Foxp3 by shRNA caused a profound induction in proliferation of responder T cells. Adoptive transfer of Foxp3(+)CD19(+) B cells attenuated the clinical symptoms of CIA significantly with concomitant suppression of IL-17 production and enhancement of Foxp3 expression in CD4(+) T cells from splenocytes. Our data indicate that Foxp3 expression is not restricted to T cells. The expression of Foxp3 in B cells is critical for the immunoregulation of T cells and limits autoimmunity in a mouse model. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 40% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 3% |
Unknown | 29 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 27% |
Researcher | 4 | 13% |
Other | 3 | 10% |
Student > Master | 3 | 10% |
Student > Doctoral Student | 1 | 3% |
Other | 2 | 7% |
Unknown | 9 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 9 | 30% |
Agricultural and Biological Sciences | 4 | 13% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Chemical Engineering | 1 | 3% |
Other | 4 | 13% |
Unknown | 9 | 30% |