Title |
Quantitative criteria for improving performance of buccal DNA for high-throughput genetic analysis
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Published in |
BMC Genomic Data, August 2012
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DOI | 10.1186/1471-2156-13-75 |
Pubmed ID | |
Authors |
Jessica G Woo, Lisa J Martin, Lili Ding, W Mark Brown, Timothy D Howard, Carl D Langefeld, Charles J Moomaw, Mary Haverbusch, Guangyun Sun, Subba R Indugula, Hong Cheng, Ranjan Deka, Daniel Woo |
Abstract |
DNA from buccal brush samples is being used for high-throughput analyses in a variety of applications, but the impact of sample type on genotyping success and downstream statistical analysis remains unclear. The objective of the current study was to determine laboratory predictors of genotyping failure among buccal DNA samples, and to evaluate the successfully genotyped results with respect to analytic quality control metrics. Sample and genotyping characteristics were compared between buccal and blood samples collected in the population-based Genetic and Environmental Risk Factors for Hemorrhagic Stroke (GERFHS) study (https://gerfhs.phs.wfubmc.edu/public/index.cfm). |
X Demographics
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United States | 1 | 50% |
Germany | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 3 | 18% |
Professor > Associate Professor | 3 | 18% |
Unspecified | 2 | 12% |
Researcher | 2 | 12% |
Student > Ph. D. Student | 2 | 12% |
Other | 3 | 18% |
Unknown | 2 | 12% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 7 | 41% |
Unspecified | 2 | 12% |
Agricultural and Biological Sciences | 2 | 12% |
Business, Management and Accounting | 1 | 6% |
Computer Science | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 18% |