Title |
Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India
|
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Published in |
BMC Microbiology, August 2012
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DOI | 10.1186/1471-2180-12-183 |
Pubmed ID | |
Authors |
Anil Kumar Verma, Ravi Verma, Vineet Ahuja, Jaishree Paul |
Abstract |
Amebic dysentery is caused by the protozoan parasite Entamoeba histolytica and the ingestion of quadrinucleate cyst of E. histolytica from fecally contaminated food or water initiates infection. Excystation occurs in the lumen of small intestine, where motile and potentially invasive trophozoites germinate from cysts. The ability of trophozoites to interact and digest gut bacteria is apparently important for multiplication of the parasite and its pathogenicity; however the contribution of resident bacterial flora is not well understood. We quantified the population of Bacteroides, Bifidobacterium, Ruminococcus, Lactobacillus, Clostridium leptum subgroup, Clostridium coccoides subgroup, Eubacterium, Campylobacter, Methanobrevibacter smithii and Sulphur reducing bacteria using genus specific primers in healthy (N = 22) vs amebic patients (E. histolytica positive, N = 17) stool samples by Real-time PCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
Czechia | 1 | <1% |
Australia | 1 | <1% |
Unknown | 105 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 20 | 19% |
Student > Ph. D. Student | 14 | 13% |
Student > Bachelor | 13 | 12% |
Student > Master | 11 | 10% |
Student > Doctoral Student | 8 | 7% |
Other | 17 | 16% |
Unknown | 25 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 30 | 28% |
Biochemistry, Genetics and Molecular Biology | 13 | 12% |
Immunology and Microbiology | 8 | 7% |
Medicine and Dentistry | 6 | 6% |
Nursing and Health Professions | 3 | 3% |
Other | 18 | 17% |
Unknown | 30 | 28% |