Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens

@jwoodgett @DanielJDrucker Also powerful synthetic lethals, while neither isoform shows a severe knockout fitness phenotype anywhere. Clear cut case of functional buffering. https://t.co/js9cHzoOsS

RT @NCIgenomics: Reconciling the low number of essential genes—and candidate cancer targets—detected in #CRISPR knockout screens by conside…

You've read her work on using enAsCas12a multiplex screens to reveal functional buffering by paralogs, a blind spot in Cas9 knockout fitness screens https://t.co/js9cHzGpRs

We next integrated all of these in a classifier to make predictions for all human paralog pairs and found that our predictions could accurately predict the results of combinatorial CRISPR screens (such as the recent work of @TraverHart and @drmervedede ht