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A prospective multicenter evaluation of direct molecular detection of blood stream infection from a clinical perspective

Overview of attention for article published in BMC Infectious Diseases, June 2016
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Title
A prospective multicenter evaluation of direct molecular detection of blood stream infection from a clinical perspective
Published in
BMC Infectious Diseases, June 2016
DOI 10.1186/s12879-016-1646-4
Pubmed ID
Authors

A. E. Nieman, P. H. M. Savelkoul, A. Beishuizen, B. Henrich, B. Lamik, C. R. MacKenzie, D. Kindgen-Milles, A. Helmers, C. Diaz, S. G. Sakka, R. P. Schade

Abstract

Rapid diagnosis and appropriate antimicrobial therapy are of major importance to decrease morbidity and mortality in patients with blood stream infections (BSI). Blood culture, the current gold standard for detecting bacteria in blood, requires at least 24-48 hours and has limited sensitivity if obtained during antibiotic treatment of the patient. The aim of this prospective multicenter study was to clinically evaluate the application of a commercial universal 16S/18S rDNA PCR, SepsiTest™ (PCR-ST), directly on whole blood. In total 236 samples from 166 patients with suspected sepsis were included in the study. PCR-ST results were compared to blood culture, the current gold standard for detecting BSI. Because blood cultures can give false-negative results, we performed an additional analysis to interpret the likelihood of bloodstream infection by using an evaluation based on clinical diagnosis, other diagnostic tests and laboratory parameters. Clinical interpretation of results defined the detected organism to be contaminants in 22 of 43 positive blood cultures (51.2 %) and 21 of 47 positive PCR-ST results (44.7 %). Excluding these contaminants resulted in an overall sensitivity and specificity of the PCR-ST of 66.7 and 94.4 % respectively. Of the 36 clinically relevant samples, 11 BSI were detected with both techniques, 15 BSI were detected with PCR-ST only and 10 with blood culture only. Therefore, in this study, SepsiTest™ detected an additional 71 % BSI compared to blood culture alone. More clinically relevant BSI were diagnosed by molecular detection, which might influence patient treatment. An improved SepsiTest™ assay suited for routine use can have additional value to blood culture in diagnosing bacteremia in septic patients.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Other 10 14%
Student > Ph. D. Student 9 12%
Researcher 9 12%
Student > Master 9 12%
Student > Bachelor 7 9%
Other 11 15%
Unknown 19 26%
Readers by discipline Count As %
Medicine and Dentistry 17 23%
Biochemistry, Genetics and Molecular Biology 14 19%
Agricultural and Biological Sciences 5 7%
Immunology and Microbiology 5 7%
Nursing and Health Professions 3 4%
Other 8 11%
Unknown 22 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2016.
All research outputs
#18,820,431
of 23,323,574 outputs
Outputs from BMC Infectious Diseases
#5,709
of 7,805 outputs
Outputs of similar age
#269,091
of 353,218 outputs
Outputs of similar age from BMC Infectious Diseases
#135
of 195 outputs
Altmetric has tracked 23,323,574 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,805 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.3. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,218 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 195 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.